Finite element 3D reconstruction of the pulmonary acinus imaged by synchrotron X-ray tomography
Finite element 3D reconstruction of the pulmonary acinus imaged by synchrotron X-ray tomography
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DOI：
10.1152/japplphysiol.90546.2008
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发表时间：
2008-09-01
影响因子：
3.3
通讯作者：
Schittny, J. C.
中科院分区：
文献类型：
--
作者：
Tsuda, A.;Filipovic, N.;Schittny, J. C.
肺腺泡的肺泡结构在气体交换功能中起着至关重要的作用。实质区域的三维（3D）分析是理解这种结构-功能关系的基础，但由于技术限制，过去只进行了有限数量的尝试。在这项研究中，我们开发了一种新的图像处理方法的基础上，有限元（FE）分析准确的三维结构重建的气体交换区域的肺。使用基于高分辨率同步加速器辐射的X射线断层摄影显微镜对体视学良好表征的大鼠肺样品（Pediatr Res 53：72-80，2003）成像。生成了1，024张图像（每个切片：1024 × 1024像素）的堆栈，每个体素的分辨率为1.4 μ m（3）。对于FE算法的开发，进一步提取包含类似于750万体素的感兴趣区域（ROI）作为工作子单元。使用基于网格的六面体算法创建覆盖体素图的3D FE。迭代地确定用于适当分割的适当阈值，以使计算的组织体积密度与体视学确定的值相匹配（Pediatr Res 53：72-80，2003）。所得到的三维有限元可以用于三维结构分析以及后续的有限元计算分析，如流体动力学和离散化。
The alveolated structure of the pulmonary acinus plays a vital role in gas exchange function. Three-dimensional (3D) analysis of the parenchymal region is fundamental to understanding this structure-function relationship, but only a limited number of attempts have been conducted in the past because of technical limitations. In this study, we developed a new image processing methodology based on finite element (FE) analysis for accurate 3D structural reconstruction of the gas exchange regions of the lung. Stereologically well characterized rat lung samples (Pediatr Res 53: 72-80, 2003) were imaged using high-resolution synchrotron radiation-based X-ray tomographic microscopy. A stack of 1,024 images (each slice: 1024 X 1024 pixels) with resolution of 1.4 mu m(3) per voxel were generated. For the development of FE algorithm, regions of interest (ROI), containing similar to 7.5 million voxels, were further extracted as a working subunit. 3D FEs were created overlaying the voxel map using a grid-based hexahedral algorithm. A proper threshold value for appropriate segmentation was iteratively determined to match the calculated volume density of tissue to the stereologically determined value (Pediatr Res 53: 72-80, 2003). The resulting 3D FEs are ready to be used for 3D structural analysis as well as for subsequent FE computational analyses like fluid dynamics and skeletonization.