Predicting ribosomal frameshifting efficiency
Predicting ribosomal frameshifting efficiency
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DOI：
10.1088/1478-3975/5/1/016002
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发表时间：
2008-03-01
期刊：
影响因子：
2
通讯作者：
Chen, Shi-Jie
中科院分区：
文献类型：
--
作者：
Cao, Song;Chen, Shi-Jie
许多逆转录病毒使用-1核糖体移码作为病毒蛋白质合成的翻译控制机制的一部分。定量预测-1移码的效率对于了解病毒基因的表达是至关重要的。在这里，我们研究了最小-1程序化核糖体框架转移机制的自由能格局，包括滑点的密码子-反密码子碱基对，下游信使RNA结构以及滑点和下游结构之间的间隔区。自由能景观分析得出了密码子-反密码子复合体移动过程中产生的张力与移码效率之间的定量关系，这种张力可能发生在A/T到A/A调节过程或移位过程中。分析表明，移码效率与下游信使核糖核酸结构的整体稳定性之间没有一致的相关性。
Many retroviruses use -1 ribosomal frameshifting as part of the mechanism in translational control of viral protein synthesis. Quantitative prediction of the efficiency of -1 frameshifting is crucial for understanding the viral gene expression. Here we investigate the free energy landscape for a minimal -1 programmed ribosomal frameshifting machinery, including the codon-anticodon base pairs at the slippery site, the downstream messenger RNA structure and the spacer between the slippery site and the downstream structure. The free energy landscape analysis leads to a quantitative relationship between the frameshifting efficiency and the tension force generated during the movement of codon-anticodon complexes, which may occur in the A/T to A/A accommodation process or the translocation process. The analysis shows no consistent correlation between frameshifting efficiency and global stability of the downstream mRNA structure.