Human Borreliosis Caused by a New World Relapsing Fever Borrelia-like Organism in the Old World
Human Borreliosis Caused by a New World Relapsing Fever Borrelia-like Organism in the Old World
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DOI：
10.1093/cid/ciy850
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发表时间：
2019-07-01
影响因子：
11.8
通讯作者：
Kawabata, Hiroki
中科院分区：
文献类型：
--
作者：
Qiu, Yongjin;Nakao, Ryo;Kawabata, Hiroki
背景复发热是非洲以前被忽视的一种传染病，给该国造成了巨大的公共卫生负担。我们的目的是调查和报告一例回归热疏螺旋体病在赞比亚。从发热患者的血液中分离出一种以前未知的疏螺旋体属物种。通过培养物分离和/或疏螺旋体特异性聚合酶链反应进行DNA检测，对疏螺旋体属种的推定载体蜱和天然宿主进行调查。利用从病人和蝙蝠标本中分离的培养物，根据基因组草图序列，通过多位点序列分析进行遗传特征分析。发热患者被诊断为回归热。在鸟类（n = 20/50 [40%]）和蝙蝠（n = 64/237 [27%]）中经常检出分离的疏螺旋体属。基于基因组序列草图的多位点序列分析显示，从患者和假定的储存宿主（蝙蝠）分离的疏螺旋体属物种形成了一个单系谱系，与在美国发现的回归热疏螺旋体属物种聚类。在赞比亚发现了一种由疏螺旋体属引起的发热性疾病，可用红霉素治疗。这是第一项报告赞比亚回归热疏螺旋体的研究，并提出了分离的疏螺旋体物种可能的天然储存宿主。有趣的是，尽管在非洲热带生态区发现了分离的疏螺旋体属物种，但它与新世界回归热疏螺旋体的关系更密切。
Background. Relapsing fever is an infectious disease previously neglected in Africa, which imposes a large public health burden in the country. We aimed to investigate and report on a case of relapsing fever borreliosis in Zambia.Methods. A previously unknown Borrelia species was isolated from the blood of a febrile patient. Investigations of the presumptive vector ticks and natural hosts for the Borrelia species were conducted by culture isolation and/or DNA detection by Borrelia-specific polymerase chain reaction. Using culture isolates from the patient and bat specimens, genetic characterization was performed by multilocus sequence analysis based on the draft genome sequences.Results. The febrile patient was diagnosed with relapsing fever. The isolated Borrelia species was frequently detected in Ornithodoros faini (n = 20/50 [40%]) and bats (n = 64/237 [27%]). Multilocus sequence analysis based on a draft genome sequence revealed that the Borrelia species isolates from the patient and presumptive reservoir host (bats) formed a monophyletic lineage that clustered with relapsing fever borreliae found in the United States.Conclusions. A febrile illness caused by a Borrelia species that was treatable with erythromycin was identified in Zambia. This is the first study to report on relapsing fever Borrelia in Zambia and suggesting the likely natural reservoir hosts of the isolated Borrelia species. Interestingly, the isolated Borrelia species was more closely related to New World relapsing fever borreliae, despite being detected in the Afrotropic ecozone.