Identification of the razor clam species Ensis arcuatus, E-siliqua, E-directus, E-macha, and Solen marginatus using PCR-RFLP analysis of the 5S rDNA region
Identification of the razor clam species Ensis arcuatus, E-siliqua, E-directus, E-macha, and Solen marginatus using PCR-RFLP analysis of the 5S rDNA region
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DOI：
10.1021/jf0709855
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发表时间：
2007-09-05
影响因子：
6.1
通讯作者：
Mendez, Josefina
中科院分区：
文献类型：
--
作者：
Fernandez-Tajes, Juan;Mendez, Josefina
应用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）技术，建立了5种梭子蟹的DNA分子标记。siliqua、长角果E. directus、直口棘球绦虫E. macha和竹蛏Solen marginatus。用5S rDNA编码区的一对通用引物进行PCR扩增。S.通过获得的扩增子的不同大小来简单地区分marginatus。用酶Hae III对E. Aesthetus，E. siliqua和E. directus，Acs I为E. macha，当两种酶结合时，也鉴定了四种。因此，这项工作提供了一个简单，可靠，快速的协议，准确识别的Ensis和竹蛏物种的新鲜和罐装产品，这是非常有用的可追溯性和执行标签法规。
Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of the 5S ribosomal DNA region has been applied to the establishment of DNA-based molecular markers for the identification of five razor clam species: Ensis arcuatus, E. siliqua, E. directus, E. macha, and Solen marginatus. PCR amplifications were carried out using a pair of universal primers from the coding region of 5S rDNA. S. marginatus was simply distinguished by the different size of the amplicons obtained. Species-specific restriction endonuclease patterns were found with the enzymes Hae III for E. arcuatus, E. siliqua, and E. directus, and Acs I for E. macha, and when two enzymes were combined, the four species were also identified. Thus, this work provides a simple, reliable, and rapid protocol for the accurate identification of Ensis and Solen species in fresh and canned products, which is very useful for traceability and to enforce labeling regulations.