Immune suppression of hematopoiesis in aplastic anemia: activity of T-gamma lymphocytes.
Immune suppression of hematopoiesis in aplastic anemia: activity of T-gamma lymphocytes.
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DOI：
10.4049/jimmunol.125.4.1449
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发表时间：
1980-10
影响因子：
4.4
通讯作者：
A. Bacigalupo;M. Podestà;M. Mingari;L. Moretta;M. V. van Lint;A. Marmont
中科院分区：
文献类型：
--
作者：
A. Bacigalupo;M. Podestà;M. Mingari;L. Moretta;M. V. van Lint;A. Marmont
本文对7例重型再生障碍性贫血（SAA）患者进行了完全自体造血重建的体外集落形成（CFU-c）研究。所有患者的集落形成均受到抑制（低于正常值的5%）。进一步的研究表明：1）去除骨髓中的T细胞可促进患者的集落形成，而去除粘附细胞（AC）则无此作用; 2）患者骨髓细胞对正常异基因骨髓的CFU-c有明显的抑制作用;（4）患者骨髓T细胞对自身及同种异体无关正常骨髓细胞的CFU-c/2均表现出明显的抑制作用，（5）1500 rads照射可使其抑制活性消失; 6）后者主要分布于骨髓T淋巴细胞中，（TG+细胞）以及培养过夜的骨髓T细胞的上清液中;（7）正常供体来源的TG+细胞对自体或同种异体骨髓细胞均无明显的CFU-c/抑制活性。因此，在缓解期SAA患者中可检测到主动骨髓抑制，并且似乎是由具有IgG受体的骨髓T细胞介导的。
In vitro colony formation (CFU-c) was studied in 7 patients with severe aplastic anemia (SAA) in complete autologous hematologic reconstitution. Colony formation was depressed in all patients (less than 5% of normal). Further studies showed that 1) patients' colony formation could be enhanced by removing T cells from the marrow, but not by removing adherent cells (AC); 2) patients' marrow cells showed a marked CFU-c inhibition against normal allogeneic marrow; 3) CFU-c/suppression could be reduced by removing T cells from the patients' marrow before the co-culture experiments; 4) patients' marrow T cells showed significant CFU-c/inhibition against autologous as well as against allogeneic unrelated normal marrow cells; 5) irradiation with 1500 rads of T cells abrogated their suppressor activity; 6) the latter was found mainly in marrow T cells with IgG receptors (TG+ cells) as well as in the supernatant of marrow T cells cultured overnight; 7) TG+ cells derived from normal donors had no significant CFU-c/inhibitory activity against autologous or allogeneic marrow cells. Active myelosuppression would thus be detectable in patients with SAA in remission and would appear to be mediated by marrow T cells with IgG receptors.