THE EFFECT OF PORE-SIZE DISTRIBUTION ON THE RATE OF ENZYMATIC-HYDROLYSIS OF CELLULOSIC SUBSTRATES

THE EFFECT OF PORE-SIZE DISTRIBUTION ON THE RATE OF ENZYMATIC-HYDROLYSIS OF CELLULOSIC SUBSTRATES
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DOI:
10.1038/nbt0285-155
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发表时间:
1985-01-01
期刊:
BIO-TECHNOLOGY
影响因子:
--
通讯作者:
GRETHLEIN, HE
GRETHLEIN, HE
中科院分区:
其他
文献类型:
--
作者:
GRETHLEIN, HE

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采用弱酸水解法对硬、软木进行预处理,并测定其孔径分布。无论底物是什么,使用来自里氏木霉的纤维素酶的水解的初始速率与可接近于51 μ m的标称直径的底物的孔体积线性相关,所述标称直径代表纤维素酶的尺寸。相反,结晶度指数与水解速率无关。
Hard and softwoods were pretreated by mild acid hydrolysis and their pore size distribution determined. Regardless of the substrate, the initial rate of hydrolysis using cellulase fromTrichoderma reeseiis linearly correlated with the pore volume of the substrate accessible to a nominal diameter of 51 Å representative of the size of the cellulase. In contrast, crystallinity index has no relationship to the rate of hydrolysis.