Surfactant protein C expression in urethane-induced murine pulmonary tumors
Surfactant protein C expression in urethane-induced murine pulmonary tumors
复制标题
DOI：
10.1016/s0002-9440(10)64717-7
复制
发表时间：
2000-01-01
影响因子：
6
通讯作者：
Shannon, JM
中科院分区：
文献类型：
--
作者：
Mason, RJ;Kalina, M;Shannon, JM
注射氨基甲酸乙酯的小鼠出现具有不同组织学模式的肿瘤，在同一肿瘤中被归类为实性、乳头状或这两种模式的混合。大多数研究人员同意实体瘤起源于肺泡型细胞，但乳头状肿瘤的细胞起源不太确定，可归因于类型II细胞或无纤毛的细支气管上皮(Clara)细胞。为了更准确地描述这些肿瘤的分化状态，并提供更多关于基因表达的信息，我们使用免疫细胞化学和/或原位杂交来确定表面活性物质相关蛋白A(SP-A)、SP-B、SP-C和SP-D、Clara细胞相关蛋白CC-10和甲状腺转录因子-1的细胞定位。在正常小鼠肺中，SP-A、SP-B和SP-D的信使RNA(MRNAs)在II型细胞和Clara细胞中均有表达。而SP-C mRNA仅在II型细胞中表达，而CC-10mRNA仅在Clara细胞中表达。所有肿瘤，无论是实体瘤还是乳头状瘤，都表达SP-A、SP-B、SP-C、SP-D和甲状腺转录因子-1，但不表达CC-10，但在较大(较老)的乳头状瘤中，SP-C的表达略有降低。这些结果表明，乌拉坦诱导的小鼠肺肿瘤表现为II型细胞表型。
Mice injected with urethane develop tumors with distinct histological patterns, which are classified as solid, papillary, or a mixture of these two patterns within the same tumor. Most investigators agree that solid tumors arise from alveolar type II cells, but the cellular origin of papillary tumors is less certain, being attributed to either type II cells or nonciliated bronchiolar epithelial (Clara) cells. To characterize the state of differentiation of these tumors more precisely and to provide additional information on gene expression, we used immunocytochemistry and/or in situ hybridization to determine the cellular localization of surfactant-associated proteins A (SP-A), SP-B, SP-C, and SP-D; Clara cell-associated protein CC-10; and thyroid transcription factor-1. In normal mouse lung, the messenger RNAs (mRNAs) for SP-A, SP-B, and SP-D were expressed in both type II cells and Clara cells. SP-C mRNA, however, was expressed only in type II cells, and CC-10 expression of mRNA was restricted to Clara cells. All tumors examined, both solid and papillary, expressed SP-A, SP-B, SP-C, SP-D, and thyroid transcription factor-1, but not CC-10, However, SP-C expression was slightly diminished in larger (older) papillary tumors. These results demonstrate that urethane-induced murine lung tumors express the type II cell phenotype.