cGMP-dependent ABA-induced stomatal closure in the ABA-insensitive Arabidopsis mutant abi1-1
cGMP-dependent ABA-induced stomatal closure in the ABA-insensitive Arabidopsis mutant abi1-1
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DOI：
10.1111/j.1469-8137.2011.03661.x
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发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
Volotovski, Igor D.
中科院分区：
文献类型：
--
作者：
Dubovskaya, Lyudmila V.;Bakakina, Yulia S.;Volotovski, Igor D.
干旱激素脱落酸（阿坝）是众所周知的，在保卫细胞气孔孔径的减少。第二信使环鸟苷3 '，本文以拟南芥野生型和ABA不敏感突变体abi 1 - 1为材料，研究了阿坝诱导气孔关闭的信号转导途径，发现cGMP是ABA诱导气孔关闭的信号转导途径的一部分。在阿坝诱导气孔关闭的信号通路中起重要作用。H2 O2和NO诱导的细胞溶质游离钙浓度（[Ca 2 +]（cyt））的增加是cGMP依赖性的，定位cGMP的[Ca 2 +]（cyt）的上游，并涉及2C型蛋白磷酸酶ABI 1的作用。cGMP的增加是通过H_2O_2和NO刺激鸟苷酸环化酶介导的。我们确定核苷二磷酸激酶作为一个新的cGMP靶蛋白在Arabidopsis。本研究的位置cGMP的ABA诱导的H_2O_2和NO的变化的下游，和上游的[Ca ~（2+）]（cyt）的信号通路导致气孔关闭。
The drought hormone abscisic acid (ABA) is widely known to produce reductions in stomatal aperture in guard cells. The second messenger cyclic guanosine 3', 5'-monophosphate (cGMP) is thought to form part of the signalling pathway by which ABA induces stomatal closure.We have examined the signalling events during cGMP-dependent ABA-induced stomatal closure in wild-type Arabidopsis plants and plants of the ABA-insensitive Arabidopsis mutant abi1-1.We show that cGMP acts downstream of hydrogen peroxide (H2O2) and nitric oxide (NO) in the signalling pathway by which ABA induces stomatal closure. H2O2- and NO-induced increases in the cytosolic free calcium concentration ([Ca2+](cyt)) were cGMP-dependent, positioning cGMP upstream of [Ca2+](cyt), and involved the action of the type 2C protein phosphatase ABI1. Increases in cGMP were mediated through the stimulation of guanylyl cyclase by H2O2 and NO. We identify nucleoside diphosphate kinase as a new cGMP target protein in Arabidopsis.This study positions cGMP downstream of ABA-induced changes in H2O2 and NO, and upstream of increases in [Ca2+](cyt) in the signalling pathway leading to stomatal closure.