The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen Verticillium dahliae.
The Transcription Factor VdHapX Controls Iron Homeostasis and Is Crucial for Virulence in the Vascular Pathogen Verticillium dahliae.
复制标题
转录因子 VdHapX 控制铁稳态并对血管病原体大丽黄萎病的毒力至关重要
DOI：
10.1128/msphere.00400-18
复制
发表时间：
2018-09-05
期刊：
影响因子：
4.8
通讯作者：
Klosterman SJ
中科院分区：
文献类型：
--
作者：
Wang Y;Deng C;Tian L;Xiong D;Tian C;Klosterman SJ
本研究表明VdHapX是一种保守的蛋白质，介导对铁饥饿和过量的适应，影响微菌核的形成，并且对大丽轮枝菌的毒力至关重要。摘要铁的体内平衡对许多病原真菌的致病力和生存力至关重要。在这里，我们表明，bZip转录因子VdHapX的功能作为一个关键的调节铁稳态的适应铁耗尽和铁过量的条件下，并需要在维管枯萎病真菌，大丽轮枝菌的全部毒力。VdHapX的缺失损害菌丝生长和分生孢子铁饥饿和铁充足。此外，VdHapX的破坏导致大丽轮枝菌的长寿命存活结构（称为微菌核）的形成减少。在缺铁条件下，ΔVdHapX菌株中参与铁利用途径和铁载体生物合成的基因表达失调。此外，ΔVdHapX菌株表现出对高铁浓度和H2O2的敏感性增加，表明VdHapX也有助于铁或H2O2解毒。ΔVdHapX菌株对烟树幼苗（黄栌）的毒力表现出强烈的降低，并且其穿透植物表皮组织的能力延迟。本研究表明VdHapX是一种保守的蛋白质，介导对铁饥饿和过量的适应，影响微菌核的形成，并且对大丽轮枝菌的毒力至关重要。
This study demonstrated that VdHapX is a conserved protein that mediates adaptation to iron starvation and excesses, affects microsclerotium formation, and is crucial for virulence of V. dahliae. ABSTRACT Iron homeostasis is essential for full virulence and viability in many pathogenic fungi. Here, we showed that the bZip transcription factor VdHapX functions as a key regulator of iron homeostasis for adaptation to iron-depleted and iron-excess conditions and is required for full virulence in the vascular wilt fungus, Verticillium dahliae. Deletion of VdHapX impaired mycelial growth and conidiation under both iron starvation and iron sufficiency. Furthermore, disruption of VdHapX led to decreased formation of the long-lived survival structures of V. dahliae, known as microsclerotia. Expression of genes involved in iron utilization pathways and siderophore biosynthesis was misregulated in the ΔVdHapX strain under the iron-depleted condition. Additionally, the ΔVdHapX strain exhibited increased sensitivity to high iron concentrations and H2O2, indicating that VdHapX also contributes to iron or H2O2 detoxification. The ΔVdHapX strain showed a strong reduction in virulence on smoke tree seedlings (Cotinus coggygria) and was delayed in its ability to penetrate plant epidermal tissue. IMPORTANCE This study demonstrated that VdHapX is a conserved protein that mediates adaptation to iron starvation and excesses, affects microsclerotium formation, and is crucial for virulence of V. dahliae.