Inheritance of two M type mitochondrial DNA from sperm and unfertilized eggs to offspring in Mytilus galloprovincialis
Inheritance of two M type mitochondrial DNA from sperm and unfertilized eggs to offspring in Mytilus galloprovincialis
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DOI：
10.1111/j.1440-169x.2007.00930.x
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发表时间：
2007-05
期刊：
影响因子：
4.6
通讯作者：
M. Obata;N. Sano;K. Kawamura;A. Komaru
中科院分区：
文献类型：
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作者：
M. Obata;N. Sano;K. Kawamura;A. Komaru
在贻贝中，来自精子的父亲线粒体DNA（mtDNA）已知会传递给后代。这种现象被称为双重单亲遗传（DUI）。在DUI下，精子mtDNA（M型）仅由男性遗传。雌性贻贝接受母体mtDNA（F型）。然而，在我们之前的研究中，我们发现雌性和未受精的卵子都有F和M型。我们假设来自精子和未受精卵的两种M类型都遗传给了后代。为了验证这一假设，我们研究了成熟的M型单倍型的数量。galloprovincialis。幼虫的M型与亲本的M型进行了比较。通过杂交试验对M型的遗传进行了研究。在20个成熟贻贝中的6个中，通过序列分析和聚合酶链反应-限制性片段长度多态性检测到两种M型。在12个杂交组合中，有5个组合的幼虫样品在单核苷酸多态性分析中出现双峰。在这些幼虫样品中，较高的峰值波与亲本M型相同。幼虫获得的父本M型远多于母本。我们证明了来自精子和未受精卵的两种M型可以在M中传递给后代。galloprovincialis。
In Mytilus mussels, paternal mitochondrial DNA (mtDNA) from sperm is known to be transmitted to offspring. This phenomenon is called doubly uniparental inheritance (DUI). Under DUI, sperm mtDNA (M type) is inherited only by males. Female mussels receive maternal mtDNA (F type). However, in our previous study, we showed female and unfertilized eggs have both F and M types. We hypothesized that the two M types both from sperm and unfertilized eggs were transmitted to offspring. To test the hypothesis, we examined the number of M type haplotypes in mature M. galloprovincialis. The M type in larvae was compared with those of the parents. Cross experiments were carried out to test the inheritance of M type. In six of 20 mature mussels, two M types were detected by sequence analysis and polymerase chain reaction‐restriction fragment length polymorphism. In cross experiments of larval samples from five of 12 crosses, double peak wave was observed by single nucleotide polymorphisms analysis. In these larval samples, the higher peak wave was identical to the parental M type. Larvae received much more paternal M type than the maternal ones. We demonstrated that two M types from sperm and unfertilized eggs were transmitted to offspring in M. galloprovincialis.