CHARACTERIZATION OF ANTIGEN-EXPRESSING PLASMODIUM-FALCIPARUM CDNA CLONES THAT ARE REACTIVE WITH PARASITE INHIBITORY ANTIBODIES
CHARACTERIZATION OF ANTIGEN-EXPRESSING PLASMODIUM-FALCIPARUM CDNA CLONES THAT ARE REACTIVE WITH PARASITE INHIBITORY ANTIBODIES
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DOI：
10.1016/0166-6851(88)90127-2
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发表时间：
1988-07-01
影响因子：
1.5
通讯作者：
INSELBURG, J
中科院分区：
文献类型：
--
作者：
HORII, T;BZIK, DJ;INSELBURG, J
恶性疟原虫（FCR3株）.lambda。 gt11cDNA表达文库由滋养体和裂殖体poly(A) RNA构建，并用来自尼日利亚的混合人免疫血清进行免疫学筛选，形成288个阳性克隆的基因库。随后使用寄生虫抑制性小鼠单克隆抗体 (mMAb) 和利比里亚人个体血清对基因库进行了筛选。两种 mMAb，43E5 和 5H10，分别与 8 个和 3 个 cDNA 克隆强烈反应。其中几个克隆也与其他 mMAb 发生微弱的交叉反应。通过 Southern 杂交，其中两个弱交叉反应克隆 cDNA#366 和 cDNA#22 显示位于寄生虫的不同染色体区域，因此似乎代表两种不同的寄生虫基因。 FCR3 和 Honduras-1 菌株中 cDNA#366 和 cDNA#22 序列的基因组组织是相同的。 cDNA#366 的核苷酸序列及其编码的氨基酸序列与先前报道的恶性疟原虫（Camp 株）外抗原（称为 p126[1]）的部分 DNA 和氨基酸序列同源。 cDNA#366 的 mRNA 似乎代表了血液阶段滋养体和裂殖体中的丰富信息。
A Plasmodium falciparum (FCR3 strain) .lambda. gt11cDNA expression library was constructed from trophozoite and schizont poly(A) RNA and was screened immunologically with a pooled human immune serum from Nigeria to form a gene bank of 288 positive clones. The gene bank was subsequently screened with parasite inhibitory mouse monoclonal antibodies (mMAb) and with individual human Liberian sera. Two mMAb, 43E5 and 5H10, strongly reacted with 8 and 3 cDNA clones, respectively. Several of those clones also weakly cross-reacted with the other mMAb. Two of those weakly cross-reactive clones, cDNA#366 and cDNA#22, were shown to be located in different chromosomal regions of the parasite by Southern hybridization and so appeared to represent two different parasite genes. The genomic organization of both cDNA#366 and cDNA#22 sequences were identical in the FCR3 and the Honduras-1 strains. The nucleotide sequence of cDNA#366 and the amino acid sequence it coded for were homologous to a partial DNA and amino acid sequence previously reported for a P. falciparum (Camp strain) exoantigen designated p126[1]. The mRNA for cDNA#366 appeared to represent an abundant message in blood stage trophozoites and schizonts.