Rice aquaporin OsPIP2;2 is a water-transporting facilitator in relevance to drought-tolerant responses.
Rice aquaporin OsPIP2;2 is a water-transporting facilitator in relevance to drought-tolerant responses.
复制标题
水稻水通道蛋白 OsPIP2;2 是与耐旱反应相关的水运输促进剂
作者：
Bai J;Wang X;Yao X;Chen X;Lu K;Hu Y;Wang Z;Mu Y;Zhang L;Dong H
在水稻（Oryza sativa）中，水通道蛋白的质膜内蛋白（PIP）家族有11个成员，OsPIP 1;1至OsPIP 1;3和OsPIP 2;1至OsPIP 2;8，其被假设促进H2O和其他小化合物跨细胞膜的运输。然而，迄今为止，只有OsPIP 1;2、OsPIP 2;1和OsPIP 2;4在其来源植物（水稻）中被证明具有底物选择性。在本研究中，OsPIP 2; 2的特征在于作为最有效的促进剂的H2O跨细胞膜运输相比，其他10 OsPIP。在所有OsPIPs的同时测试中，四个基因（OsPIP 1;3、OsPIP 2;1、OsPIP 2;2和OsPIP 2;4）在生理干旱胁迫后的水稻植株的叶片中被诱导表达，其中OsPIP 2;2表达量最高。在青蛙卵母细胞和酵母细胞中重新表达后，4种OsPIP蛋白均以三聚体和四聚体的形式定位于质膜，并显示出增加质膜透性的活性。相比之下，OsPIP 2;2最支持H2O进入卵母细胞和酵母细胞。在烟草原生质体中从头表达后，OsPIP 2;2超过OsPIP 1;3、OsPIP 2;1和OsPIP 2;4来支持H2O跨质膜运输。OsPIP2; 2介导的H2O运输伴随着耐旱反应，包括脯氨酸和多胺浓度的增加，这两者都是耐旱性的生理标志。在水稻原生质体中，OsPIP 2;2过表达显著增强了H2O的运输和耐旱反应，包括耐旱途径标记基因的表达，但基因沉默强烈抑制了这些反应。OsPIP2; 2具有维持细胞膜完整性的作用，能有效地保护水稻细胞免受生理干旱胁迫引起的电解质渗漏。这些结果表明，OsPIP 2;2是植物耐旱性相关的H2O运输的主要促进剂。
In rice (Oryza sativa), the PLASMA MEMBRANE INTRINSIC PROTEIN (PIP) family of aquaporin has 11 members, OsPIP1;1 to OsPIP1;3, and OsPIP2;1 to OsPIP2;8, which are hypothesized to facilitate the transport of H2O and other small compounds across cell membranes. To date, however, only OsPIP1;2, OsPIP2;1, and OsPIP2;4 have been demonstrated for substrate selectivity in their source plant (rice). In this study, OsPIP2;2 was characterized as the most efficient facilitator of H2O transport across cell membranes in comparison with the other 10 OsPIPs. In concomitant tests of all OsPIPs, four genes (OsPIP1;3, OsPIP2;1, OsPIP2;2, and OsPIP2;4) were induced to express in leaves of rice plants following a physiological drought stress, while OsPIP2;2 was expressed to the highest level. After de novo expression in frog oocytes and yeast cells, the four OsPIP proteins were localized to the plasma membranes in trimer and tetramer and displayed the activity to increase the membrane permeability to H2O. In comparison, OsPIP2;2 was most supportive to H2O import to oocytes and yeast cells. After de novo expression in tobacco protoplasts, OsPIP2;2 exceeded OsPIP1;3, OsPIP2;1, and OsPIP2;4 to support H2O transport across the plasma membranes. OsPIP2;2‐mediated H2O transport was accompanied by drought‐tolerant responses, including increases in concentrations of proline and polyamines, both of which are physiological markers of drought tolerance. In rice protoplasts, H2O transport and drought‐tolerant responses, which included expression of marker genes of drought tolerance pathway, were considerably enhanced by OsPIP2;2 overexpression but strongly inhibited by the gene silencing. Furthermore, OsPIP2;2 played a role in maintenance of the cell membrane integrity and effectively protected rice cells from electrolyte leakage caused by the physiological drought stress. These results suggest that OsPIP2;2 is a predominant facilitator of H2O transport in relevance to drought tolerance in the plant.
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发表时间：
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