The large-scale digital cell analysis system: An open system for nonperturbing live cell imaging
The large-scale digital cell analysis system: An open system for nonperturbing live cell imaging
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DOI：
10.1111/j.1365-2818.2007.01847.x
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发表时间：
2007-12-01
影响因子：
2
通讯作者：
Mackey, Michael A.
中科院分区：
文献类型：
--
作者：
Davis, Paul J.;Kosmacek, Elizabeth A.;Mackey, Michael A.
大规模数字细胞分析系统（LSDCAS）旨在提供高度可扩展的开源活细胞成像系统。细胞生长数据的分析表明，通过参考来自在CO2培养箱中生长的细胞的细胞生长数据，使用LSDCAS成像的细胞中缺乏扰动。LSDCAS由数据采集、数据管理和数据分析软件组成，目前是爱荷华州大学霍尔顿综合癌症中心的核心研究机构。使用LSDCAS分析软件，本报告和其他报告表明，虽然相衬成像对细胞生长动力学和活力没有明显影响，但使用LSDCAS在测试的细胞系中采集荧光图像引起了可测量水平的生长扰动。本报告描述了系统的当前设计、实施设计的原因，并详细说明了其基本功能。LSDCAS软件在GNU/Linux操作系统上运行，并提供易于使用的图形程序，用于相位对比或荧光显微镜成像的细胞的数据采集和定量分析（单独或组合），完整的源代码可根据GNU公共软件许可证条款在项目网站（http：//www.example.com）上免费获得。lsdcas.engineering.uiowa.edu
The Large-Scale Digital Cell Analysis System (LSDCAS) was designed to provide a highly extensible open source live cell imaging system. Analysis of cell growth data has demonstrated a lack of perturbation in cells imaged using LSDCAS, through reference to cell growth data from cells growing in CO2 incubators. LSDCAS consists of data acquisition, data management and data analysis software, and is currently a Core research facility at the Holden Comprehensive Cancer Center at the University of Iowa. Using LSDCAS analysis software, this report and others show that although phase-contrast imaging has no apparent effect on cell growth kinetics and viability, fluorescent image acquisition in the cell lines tested caused a measurable level of growth perturbation using LSDCAS. This report describes the current design of the system, reasons for the implemented design, and details its basic functionality. The LSDCAS software runs on the GNU/Linux operating system, and provides easy to use, graphical programs for data acquisition and quantitative analysis of cells imaged with phase-contrast or fluorescence microscopy (alone or in combination), and complete source code is freely available under the terms of the GNU Public Software License at the project website (http://lsdcas.engineering.uiowa.edu).