Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus
Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus
复制标题
DOI：
10.1016/s0014-5793(96)01232-x
复制
发表时间：
1996-12-02
期刊：
影响因子：
3.5
通讯作者：
Golvano, MP
中科院分区：
文献类型：
--
作者：
Cueto, M;HernandezPerera, O;Golvano, MP
NO是一种广泛存在于生理学中的信使分子，我们对植物中是否存在NO产生系统进行了研究。白羽扇豆（Lupinus albus）根和根瘤以L-精氨酸依赖的方式合成NO和L[C-14]瓜氨酸。L-[C-14]瓜氨酸的产生被一氧化氮合酶拮抗剂N-G-单甲基-L-精氨酸以竞争性方式抑制，NADPH-黄递酶活性定位于根和根瘤的维管束中，并且也定位于根瘤感染区中。在N-G-单甲基-L-精氨酸存在下，这种染色显著减少。这些结果表明植物中存在假定的一氧化氮合酶。
NO is a widespread messenger molecule in physiology, We were interested in investigating whether an NO-generating system could be present in plants, NO and L[C-14]citrulline were synthesized by roots and nodules of Lupinus albus in an L-arginine-dependent manner. L-[C-14]Citrulline production was inhibited by N-G-monomethyl-L-arginine, a nitric oxide synthase antagonist, in a competitive way, NADPH-diaphorase activity was localized in the vascular bundles in root and nodules, and also in the nodule infected zone, This staining was significantly reduced in the presence of N-G-monomethyl-L-arginine, These results indicate the presence of a putative nitric oxide synthase in plants.