Comparative Proteomic Analysis of Two Barley Cultivars (Hordeum vulgare L.) with Contrasting Grain Protein Content.
Comparative Proteomic Analysis of Two Barley Cultivars (Hordeum vulgare L.) with Contrasting Grain Protein Content.
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两个大麦品种 (Hordeum vulgare L.) 谷物蛋白质含量对比的蛋白质组学分析
DOI：
10.3389/fpls.2016.00542
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发表时间：
2016
影响因子：
5.6
通讯作者：
Xu R
中科院分区：
文献类型：
--
作者：
Guo B;Luan H;Lin S;Lv C;Zhang X;Xu R
大麦种子的谷物蛋白质含量 (GPC) 在饲料大麦品种和啤酒大麦品种之间存在显着差异。然而，目前还没有深入了解饲料大麦品种和啤酒大麦品种之间种子蛋白的蛋白质组学分析。此外，大麦 GPC 的遗传控制仍不清楚。测量了扬四麦 3 号和 Naso Nijo 成熟籽粒的 GPC。通过结合使用 2-DE 和串联质谱法，建立了差异表达蛋白质的蛋白质组分析。在大麦种子蛋白质组中总共检测到 502 个可重复的蛋白质点，pH 范围为 4-7 和 6-11，其中 41 个蛋白质点（8.17%）在阳司麦 3 号和 Naso Nijo 之间检测到差异表达。鉴定出对应23种不同蛋白质的34个蛋白质点，分为八类，包括应激、蛋白质降解和翻译后修饰、发育、细胞、信号传导、糖酵解、淀粉代谢和其他功能。在已鉴定的蛋白质中，烯醇化酶（点274）和ADP-葡萄糖焦磷酸化酶小亚基（点271）仅在大麦Yangsimai 3中表达，其可能参与调节种子蛋白质表达。此外，麦芽质量的特征是丝氨酸蛋白酶抑制剂蛋白、α-淀粉酶/胰蛋白酶抑制剂CMb和α-淀粉酶抑制剂BDAI-1的积累。最值得注意的是，球蛋白是大麦种子中的一种重要储存蛋白，在两个品种中都经历翻译后加工，并且还表现出不同的表达模式。
Grain protein contents (GPCs) of barley seeds are significantly different between feed and malting barley cultivars. However, there is still no insight into the proteomic analysis of seed proteins between feed and malting barley cultivars. Also, the genetic control of barley GPC is still unclear. GPCs were measured between mature grains of Yangsimai 3 and Naso Nijo. A proteome profiling of differentially expressed protein was established by using a combination of 2-DE and tandem mass spectrometry. In total, 502 reproducible protein spots in barley seed proteome were detected with a pH range of 4–7 and 6–11, among these 41 protein spots (8.17%) were detected differentially expressed between Yangsimai 3 and Naso Nijo. Thirty-four protein spots corresponding to 23 different proteins were identified, which were grouped into eight categories, including stress, protein degradation and post-translational modification, development, cell, signaling, glycolysis, starch metabolism, and other functions. Among the identified proteins, enolase (spot 274) and small subunit of ADP-glucose pyrophosphorylase (spot 271) are exclusively expressed in barley Yangsimai 3, which may be involved in regulating seed protein expression. In addition, malting quality is characterized by an accumulation of serpin protein, Alpha-amylase/trypsin inhibitor CMb and Alpha-amylase inhibitor BDAI-1. Most noticeably, globulin, an important storage protein in barley seed, undergoes post-translational processing in both cultivars, and also displays different expression patterns.