Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA
Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA
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DOI：
10.1073/pnas.1103411108
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发表时间：
2011-05-24
影响因子：
11.1
通讯作者：
Knipe, David M.
中科院分区：
文献类型：
--
作者：
Chang, Lynne;Godinez, William J.;Knipe, David M.
染色质核内运动在基因表达中的作用尚不清楚。单纯疱疹病毒在感染细胞核中形成复制区室（RCs），作为病毒DNA复制和晚期基因转录的位点。这些结构由小的隔室发展而来，这些隔室在大小、移动和结合上不断增长。从4D图像中导出的RC轨迹定量分析表明，大多数RC是定向运动的。定向运动在肌动蛋白和肌球蛋白抑制剂以及转录抑制剂的存在下受损。此外，RCs在核斑点处聚并重组。最后，肌动蛋白和肌球蛋白抑制剂对病毒基因表达的不同影响表明，转录不需要RC运动，相反，运动导致转录活性RC与核斑点桥接，形成增强病毒晚期mrna输出的结构。
The role of the intranuclear movement of chromatin in gene expression is not well-understood. Herpes simplex virus forms replication compartments (RCs) in infected cell nuclei as sites of viral DNA replication and late gene transcription. These structures develop from small compartments that grow in size, move, and coalesce. Quantitative analysis of RC trajectories, derived from 4D images, shows that most RCs move by directed motion. Directed movement is impaired in the presence of actin and myosin inhibitors as well as a transcription inhibitor. In addition, RCs coalesce at and reorganize nuclear speckles. Lastly, distinct effects of actin and myosin inhibitors on viral gene expression suggest that RC movement is not required for transcription, but rather, movement results in the bridging of transcriptionally active RCs with nuclear speckles to form structures that enhance export of viral late mRNAs.