Childhood Obesity and the Role of Dopamine D2 Receptor and Cannabinoid Receptor-1 Gene Polymorphisms
Childhood Obesity and the Role of Dopamine D2 Receptor and Cannabinoid Receptor-1 Gene Polymorphisms
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DOI：
10.1089/gtmb.2012.0244
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发表时间：
2012-12-01
影响因子：
1.4
通讯作者：
Aynacioglu, A. Sukru
中科院分区：
文献类型：
--
作者：
Araz, Nilgun Col;Nacak, Muradiye;Aynacioglu, A. Sukru
目的：多巴胺能和内源性大麻素系统参与摄食行为的调节。该研究的目的是研究多巴胺D2受体（DRD 2）和大麻素受体-1（CNR 1）基因多态性与儿童肥胖之间的可能关系。方法：对100名肥胖儿童和100名健康对照者进行DRD 2 Taq 1A、Taq 1B和CNR 1 1359 G/A基因多态性分析。采用聚合酶链反应和限制性片段长度多态性进行基因分型。结果如下：DRD 2 Taq 1A和DRD 2 Taq 1B基因型和等位基因频率在肥胖儿童和对照组之间差异无统计学意义（p > 0.05）。在Taq 1B 2等位基因患者中，病态肥胖发生率较低（p = 0.010）。肥胖儿童CNR 1 1359 G/A多态性A等位基因的频率显着高于对照组（21.0%比13.0%，p = 0.0166）。CNR 1 1359 G/A SNP的基因型AG和GG的频率在肥胖儿童和对照受试者之间是不同的（AG：34.0%对22.0%，p = 0.0294; GG：62.0%对76.0%，p = 0.0162）。结论：DRD 2 Taq 1A和DRD 2 Taq 1B基因型和等位基因在肥胖组和对照组间差异无统计学意义，而CNR 1受体1359 G/A多态性和A等位基因的存在可能是肥胖易感性的危险因素之一。
Aims: The dopaminergic and endocannabinoid systems are involved in regulation of feeding behavior. The aim of the study is to examine the possible relation between polymorphisms of the dopamine D2 receptor (DRD2) and cannabinoid receptor-1 (CNR1) genes and childhood obesity. Methods: A hundred obese children and 100 healthy controls were analyzed for DRD2 Taq1A and Taq1B and CNR1 1359G/A polymorphisms. Genotyping was performed by polymerase chain reaction and restriction fragment length polymorphism. Results: There were no statistically significant differences in DRD2 Taq1A and DRD2 Taq1B genotypes or allelic frequencies between obese children and controls (p > 0.05). In patients with Taq1B2 allele, morbid obesity was less frequent (p = 0.010). The frequency of the A allele of CNR1 1359G/A polymorphism was significantly higher in obese children than in controls (21.0% vs. 13.0%, p = 0.0166). The frequency of genotypes AG and GG of the CNR1 1359G/A SNP was different between obese children and control subjects (for AG: 34.0% vs. 22.0%, p = 0.0294; for GG: 62.0% vs. 76.0%, p = 0.0162, respectively). Conclusions: No significant difference was found between genotypes and alleles of DRD2 Taq1A and DRD2 Taq1B polymorphism in patients and controls, while the CNR1 receptor 1359G/A polymorphism and the presence of the A allele may be one risk factor for susceptibility to obesity.