A BIOSENSOR CONCEPT BASED ON IMAGING ELLIPSOMETRY FOR VISUALIZATION OF BIOMOLECULAR INTERACTIONS
A BIOSENSOR CONCEPT BASED ON IMAGING ELLIPSOMETRY FOR VISUALIZATION OF BIOMOLECULAR INTERACTIONS
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DOI：
10.1006/abio.1995.9959
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发表时间：
1995-11-20
影响因子：
2.9
通讯作者：
ARWIN, H
中科院分区：
文献类型：
--
作者：
JIN, G;TENGVALL, P;ARWIN, H
提出了一种新型的光学生物传感器系统，并将其应用于检测硅衬底上的抗原抗体复合物。生物传感器系统利用生物分子相互作用的特异性与蛋白质图案化表面组合。通过使用成像椭圆偏振法实现了表面上薄层（蛋白质图案）的厚度分布的可视化。该技术使用CCD照相机，由此可以进行大表面的椭圆偏振分析。该传感器系统具有空间分辨率高、数据采集速度快、使用简单等优点。(C)出版社：Academic Press
A new type of optical biosensor system is proposed and its application to detection of antigen-antibody complexes on a silicon substrate is demonstrated. The biosensor system is utilizing specificities of biomolecular interactions in combination with protein patterned surfaces. Visualization of the thickness distributions of thin layers (protein patterns) on a surface is achieved by using imaging ellipsometry. This technique uses a CCD camera whereby an ellipsometric analysis of a large surface can be made. The biosensor system has the advantages of high spatial resolution, fast data acquisition, and simplicity in use. (C) 1995 Academic Press, Inc.