Infection density of Wolbachia endosymbiont affected by co-infection and host genotype
Infection density of Wolbachia endosymbiont affected by co-infection and host genotype
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DOI：
10.1098/rsbl.2005.0340
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发表时间：
2005-12-22
期刊：
影响因子：
3.3
通讯作者：
Fukatsu, T
中科院分区：
文献类型：
--
作者：
Kondo, N;Shimada, M;Fukatsu, T
感染密度是了解Wolbachia和其他内共生体对其宿主的生物学效应的最重要因素之一。为了深入了解侵染密度调控的机制，我们对赤豆天牛及其Wolbachia内共生菌进行了研究。双重感染，单感染和未感染的宿主菌株与控制核遗传背景产生的渗入，并在这些菌株的感染密度进行了评价，通过定量聚合酶链反应技术。我们的研究揭示了沃尔巴克氏体密度调节的以前未知的方面：（i）识别不同影响沃尔巴克氏体密度的特定宿主基因型和（ii）通过共感染沃尔巴克氏体菌株抑制沃尔巴克氏体密度。这些研究结果揭示了新的光共生体和主机共生体的相互作用在沃尔巴克氏体昆虫内共生，并强烈建议，沃尔巴克氏体密度是通过一个复杂的相互作用之间的宿主基因型，共生体基因型和其他因素。
Infection density is among the most important factors for understanding the biological effects of Wolbachia and other endosymbionts on their hosts. To gain insight into the mechanisms of infection density regulation, we investigated the adzuki bean beetles Callosobruchus chinensis and their Wolbachia endosymbionts. Double-infected, single-infected and uninfected host strains with controlled nuclear genetic backgrounds were generated by introgression, and infection densities in these strains were evaluated by a quantitative polymerase chain reaction technique. Our study revealed previously unknown aspects of Wolbachia density regulation: (i) the identification of intra-specific host genotypes that affect Wolbachia density differently and (ii) the suppression of Wolbachia density by co-infecting Wolbachia strains. These findings shed new light on symbiont-symbiont and host-symbiont interactions in the Wolbachia-insect endosymbiosis and strongly suggest that Wolbachia density is determined through a complex interaction between host genotype, symbiont genotype and other factors.