A translationally controlled tumor protein gene Rpf41 is required for the nodulation of Robinia pseudoacacia
A translationally controlled tumor protein gene Rpf41 is required for the nodulation of Robinia pseudoacacia
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刺槐结瘤需要翻译控制的肿瘤蛋白基因 Rpf41
DOI：
10.1007/s11103-015-0424-9
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发表时间：
2016-03-01
影响因子：
5.1
通讯作者：
Wei, Gehong
中科院分区：
文献类型：
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作者：
Chou, Minxia;Xia, Congcong;Wei, Gehong
翻译控制肿瘤蛋白（TCTP）是真核生物发育和一般生长调节的基础。其多种功能已从其参与多种细胞途径中推断出来，但不能先验地暗示其在豆类共生结瘤中的潜在参与。在目前的工作中，我们从木本豆科树刺槐中鉴定并表征了 TCTP 的同源物 Rpf41，该基因在接种后 15 天在受感染根中表达上调，但在成熟根瘤中表达下降。亚细胞定位实验表明Rpf41蛋白定位于质膜、细胞质、细胞核，也可能定位于细胞骨架。通过 RNA 干扰 (RNAi) 敲低 Rpf41 会导致根瘤和根毛的发育受损。与野生植物相比，Rpf41 RNAi植物的根茎长度、鲜重和单株根瘤数显着降低。 Rpf41 RNAi 根中 IT 或根瘤原基的数量也显着减少。结节超微结构分析显示，Rpf41 RNAi结节中感染细胞发育仍保留在II区，该区感染细胞较少。此外，在 Rpf41 RNAi 结节的感染细胞中，共生体表现出明显的菌体收缩和菌体周围空间扩大。在较深的细胞层中，观察到感染细胞超微结构的更显着畸变，并检测到类菌细胞质中的电子透明病变。这些结果首次证实TCTP是豆科植物共生结瘤的重要调节因子，并且可能参与刺槐共生细胞分化和防止幼瘤过早衰老。
Translationally controlled tumor protein (TCTP) is fundamental for the regulation of development and general growth in eukaryotes. Its multiple functions have been deduced from its involvement in several cell pathways, but its potential involvement in symbiotic nodulation of legumes cannot be suggested a priori. In the present work, we identified and characterized from the woody leguminous tree Robinia pseudoacacia a homolog of TCTP, Rpf41, which was up-regulated in the infected roots at 15 days post-inoculation but decreased in the matured nodules. Subcellular location assay showed that Rpf41 protein was located in the plasma membrane, cytoplasm, nucleus, and also maybe in cytoskeleton. Knockdown of Rpf41 via RNA interference (RNAi) resulted in the impaired development of both nodule and root hair. Compared with wild plants, the root and stem length, fresh weight and nodule number per plant was decreased dramatically in Rpf41 RNAi plants. The number of ITs or nodule primordia was also signficantly reduced in the Rpf41 RNAi roots. The analyses of nodule ultrastructure showed that the infected cell development in Rpf41 RNAi nodules remained in zone II, which had fewer infected cells. Furthermore, the symbiosomes displayed noticeable shrinkage of bacteroid and peribacteroid space enlargement in the infected cells of Rpf41 RNAi nodules. In the deeper cell layers, a more remarkable aberration of the infected cell ultrastructure was observed, and electron-transparent lesions in the bacteroid cytoplasm were detected. These results identify TCTP as an important regulator of symbiotic nodulation in legume for the first time, and it may be involved in symbiotic cell differentiation and preventing premature aging of the young nodules in R. pseudoacacia.