Effect of L-dopa on interleukin-1 beta-induced suppression of luteinizing hormone secretion in intact female rats.
Effect of L-dopa on interleukin-1 beta-induced suppression of luteinizing hormone secretion in intact female rats.
复制标题
左旋多巴对白细胞介素 1 β 诱导的完整雌性大鼠黄体生成素分泌抑制的影响。
DOI：
10.1093/humrep/den434
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
MohanKumar,SMJ
中科院分区：
文献类型：
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作者：
Sirivelu,MP;Shin,AC;Perez,GI;MohanKumar,PS;MohanKumar,SMJ
细胞因子白介素-1β (IL-1β)在免疫应激时增加，并且已知通过降低下丘脑去甲肾上腺素（NE）来抑制雌性大鼠排卵前黄体生成素（LH）的激增。我们假设IL-1β可能通过减少NE的生物合成来产生这种作用。方法雌性spraguedawley大鼠下丘脑内侧视前区（MPA）置入推拉式插管，颈静脉置入导管。在发情当天的1300小时，分别给予5µg IL-1β、NE前体、左旋多巴或左旋多巴和IL-1β的混合物。进行推拉灌注和连续采血。分析灌注液中NE水平和血清中LH水平。结果sil -1β抑制了MPA中NE水平的升高和LH水平的升高。左旋多巴治疗能够部分恢复发情前下午的NE和LH水平。IL-1β治疗导致排卵失败，左旋多巴也能逆转这一作用。结论IL-1β可通过降低雌性大鼠MPA内NE水平来抑制生殖功能，而左旋多巴可拮抗这一作用。
BACKGROUNDThe cytokine, interleukin-1β (IL-1β), increases during immune stress and is known to suppress the preovulatory luteinizing hormone (LH) surge in female rats by decreasing hypothalamic norepinephrine (NE). We hypothesized that IL-1β could produce this effect by decreasing NE biosynthesis.METHODSFemale Sprague–Dawley rats were implanted with a push–pull cannula in the medial preoptic area (MPA) of the hypothalamus and a catheter in the jugular vein. They were treated i.p. with the vehicle or 5 µg of IL-1β, the NE precursor, L-dopa, or a combination of L-dopa and IL-1β at 1300 hours on the day of proestrus. They were subjected to push–pull perfusion and serial blood sampling. Perfusates were analyzed for NE levels and serum samples for LH.RESULTSIL-1β treatment blocked the increase in NE levels in the MPA and the LH surge. Treatment with L-dopa was able to partially restore both NE and LH levels during the afternoon of proestrus. IL-1β treatment caused failure of ovulation and this effect was also reversed by L-dopa.CONCLUSIONSThese results suggest that IL-1β could decrease NE levels in the MPA to suppress reproductive functions and L-dopa can be used to counter this effect.