SELECTIVE ENRICHMENT OF CHOLINERGIC NEURONS WITH THE ALPHA-KETOGLUTARATE DEHYDROGENASE COMPLEX IN RAT-BRAIN
SELECTIVE ENRICHMENT OF CHOLINERGIC NEURONS WITH THE ALPHA-KETOGLUTARATE DEHYDROGENASE COMPLEX IN RAT-BRAIN
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DOI：
10.1016/0304-3940(94)90452-9
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发表时间：
1994-02-28
影响因子：
2.5
通讯作者：
GIBSON, GE
中科院分区：
文献类型：
--
作者：
CALINGASAN, NY;BAKER, H;GIBSON, GE
大量报告表明乙酰胆碱内稳态和氧化代谢之间存在密切相互作用。然而，这种关系的神经解剖学基础尚未确立。先前的一项研究显示，一种关键的线粒体酶——α - 酮戊二酸脱氢酶复合物（KGDHC）在大鼠整个大脑的神经元、神经胶质和神经毡中含量较低。一些区域，包括富含胆碱能神经元标志物胆碱乙酰转移酶（ChAT）的区域，显示出KGDHC在胞体周围相对较高的富集程度。当前的研究利用双标记免疫荧光法来确定大鼠大脑中胆碱能神经元是否富含KGDHC。在脑神经核、斜方体核、疑核和下橄榄核中，几乎所有胆碱能神经元都富含KGDHC。然而，在基底前脑核中，只有一部分胆碱能细胞对KGDHC呈强免疫反应。这些数据提供了形态学证据，支持了特定脑区中胆碱能功能与氧化代谢之间假设的联系。
Numerous reports suggest a close interaction between acetylcholine homeostasis and oxidative metabolism. However, the neuroanatomical basis of this relationship has not been established. A previous study showed that a key mitochondrial enzyme, alpha-ketoglutarate dehydrogenase complex (KGDHC) occurs at low levels in neurons, glia and neuropil throughout the rat brain. Some regions including those that are enriched with a cholinergic neuronal marker, choline acetyltransferase (ChAT) show relatively high perikaryal enrichment of KGDHC. The current study utilized double label immunofluorescence to determine whether cholinergic neurons are enriched with KGDHC in rat brain. In cranial nerve nuclei, trapezoid nucleus, nucleus ambiguus and inferior olive, virtually all cholinergic neurons were enriched with KGDHC. However, in basal forebrain nuclei, only a subpopulation of cholinergic cells were intensely immunoreactive for KGDHC. These data provide morphological evidence to support the hypothesized link between cholinergic function and oxidative metabolism in specific brain regions.