Modulation of ATP-induced inward currents by docosahexaenoic acid and other fatty acids in rat nodose ganglion neurons
Modulation of ATP-induced inward currents by docosahexaenoic acid and other fatty acids in rat nodose ganglion neurons
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DOI：
10.1254/jphs.sc0060053
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发表时间：
2006-11-01
影响因子：
3.5
通讯作者：
Ishibashi, Hitoshi
中科院分区：
文献类型：
--
作者：
Eto, Kei;Arimura, Yukiko;Ishibashi, Hitoshi
应用制霉菌素穿孔膜片钳技术，观察了二十二碳六烯酸(DHA)和其他脂肪酸对大鼠结状神经节细胞P2X受体介导的内向电流的影响。DHA加速了ATP诱导电流的脱敏速度，DHA呈剂量依赖性抑制ATP诱导电流的峰值。其他多不饱和脂肪酸，如花生四烯酸和二十碳五烯酸，也表现出类似的使用依赖抑制。棕榈酸和花生四烯酸等饱和脂肪酸的抑制作用弱于多不饱和脂肪酸。结果提示，当通道处于开放状态时，脂肪酸可能参与调节P2X受体介导的反应。
The effects of docosahexaenoic acid (DHA) and other fatty acids on P2X-receptor-mediated inward currents in rat nodose ganglion neurons were studied using the nystatin perforated patch-clamp technique. DHA accelerated the desensitization rate of the ATP-induced current, DHA showed use-dependent inhibition of the peak ATP-induced current. Other polyunsaturated fatty acids, such as arachidonic acid and eicosapentaenoic acid, displayed a similar use-dependent inhibition. The inhibitory effects of saturated fatty acids including palmitic acid and arachidic acid were weaker than those of polyunsaturated fatty acids. The results suggest that fatty acids may modulate the P2X receptor mediated response when the channel is in the open-state.