Redox-active metal-containing nucleotides: synthesis, tunability, and enzymatic incorporation into DNA.
Redox-active metal-containing nucleotides: synthesis, tunability, and enzymatic incorporation into DNA.
复制标题
含氧化还原活性金属的核苷酸：合成、可调性和酶促掺入 DNA。
DOI：
10.1021/ja017193q
复制
发表时间：
2002
影响因子：
15
通讯作者：
Tor,Yitzhak
中科院分区：
文献类型：
--
作者：
Weizman,Haim;Tor,Yitzhak
使用（a）双取代的Ru 2+或Os 2+前体（R2 bpy）2 ML 2，（b）取代的2，4-戊二酮或带有功能化接头的异羟肟酸，和（B）模块化合成具有可调电化学电位的新型氧化还原活性DNA标记标签，以及（c）修饰的核苷酸。DNA聚合酶有效地将含金属的核苷酸三磷酸掺入DNA寡核苷酸中。
Novel redox-active DNA labeling tags with tunable electrochemical potentials are modularly synthesized using (a) bis-substituted Ru2+or Os2+precursors (R2bpy)2ML2, (b) substituted 2,4-pentanediones or hydroxamic acids bearing a functionalized linker, and (c) modified nucleotides. DNA polymerase efficiently incorporates the metal-containing nucleotide triphosphate into DNA oligonucleotides.