Barley Ant17, encoding flavanone 3-hydroxylase (F3H), is a promising target locus for attaining anthocyanin/proanthocyanidin-free plants without pleiotropic reduction of grain dormancy
Barley Ant17, encoding flavanone 3-hydroxylase (F3H), is a promising target locus for attaining anthocyanin/proanthocyanidin-free plants without pleiotropic reduction of grain dormancy
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DOI：
10.1139/gen-2014-0189
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发表时间：
2015-01-01
期刊：
影响因子：
3.1
通讯作者：
Taketa, Shin
中科院分区：
文献类型：
--
作者：
Himi, Eiko;Taketa, Shin
收获前发芽是粮食作物生产中的一个严重问题，它会造成粮食作物品质恶化和经济损失。众所周知，小麦的籽粒颜色与籽粒休眠密切相关；没有积累原花青素的白粒系比红粒系更容易在收获前发芽。所有可用的白粒小麦品系都局限于R位点（R- a1， R- b1和R- d1）的三隐性突变，但大麦已知有11个独立的位点赋予无原花青素的谷物表型。在这项研究中，我们评估了花青素/不含原花青素的ant17突变体的休眠水平。三种突变的蚂蚁表现出与其各自的野生型相同的休眠水平。对三个独立的ant17等位基因进行测序，发现黄烷酮-3-羟化酶（F3H）编码区存在点突变，预计这将导致不同位点的过早停止密码子。F3H位点与染色体臂2HL上的Ant17位点完全共分离。F3H基因在大麦色素组织中表达，而在非色素组织中不表达。这一结果表明，小麦F3H可能是选育具有实用抗采前发芽能力的白粒系的新靶点。
Preharvest sprouting is a serious problem in grain crop production because it causes quality deterioration and economic losses. It is well known that grain colour is closely associated with grain dormancy in wheat; white-grained lines without accumulating proanthocyanidins in testa tend to be more susceptible to preharvest sprouting than red ones. All available white-grained wheat lines are restricted to triple recessive mutations at the R loci (R-A1, R-B1, and R-D1), but barley is known to have 11 independent loci conferring the proanthocyanidin-free grain phenotype. In this study, we evaluated the dormancy levels of anthocyanin/proanthocyanidin-free ant17 mutants. Three ant17 mutants showed the same levels of dormancy as their respective wild types. Sequencing of three independent ant17 alleles detected a point mutation within the coding regions of flavanone-3-hydroxylase (F3H), which are predicted to cause a premature stop codon at different sites. The F3H locus completely cosegregated with the Ant17 position on the chromosome arm 2HL. Expression of the barley F3H gene was observed in pigmented tissues, but not in nonpigmented roots and stems. This result indicates that wheat F3H may be a promising new target locus for breeding white-grained lines with a practical level of preharvest sprouting resistance.