Progress in the structural and functional characterization of kinetochores
Progress in the structural and functional characterization of kinetochores
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DOI：
10.1016/j.sbi.2016.03.003
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发表时间：
2016-04-01
影响因子：
6.8
通讯作者：
Musacchio, Andrea
中科院分区：
文献类型：
--
作者：
Pesenti, Marion E.;Weir, John R.;Musacchio, Andrea
着丝粒是一种大分子复合物，建立在一个称为着丝粒的特殊染色质结构域上。着丝粒在有丝分裂过程中为纺锤体微管提供了一个附着点。它们还控制细胞周期检查点，纺锤体组装检查点，其协调有丝分裂退出与有丝分裂纺锤体上染色体对齐的完成。因此正确的着丝粒操作对染色体的准确分离是不可缺少的。着丝粒是已知最大的大分子机器之一，它至少有30个结构核心亚基和无数的调节亚基。生化重组和结构分析，以及功能研究，正在揭示这些复杂而迷人的结构的组织原理。我们总结了最近的工作，并确定了未来工作的一些挑战。
Kinetochores are macromolecular complexes built on a specialized chromatin domain called the centromere. Kinetochores provide a site of attachment for spindle microtubules during mitosis. They also control a cell cycle checkpoint, the spindle assembly checkpoint, which coordinates mitotic exit with the completion of chromosome alignment on the mitotic spindle. Correct kinetochore operation is therefore indispensable for accurate chromosome segregation. With multiple copies of at least 30 structural core subunits and a myriad of regulatory subunits, kinetochores are among the largest known macromolecular machines. Biochemical reconstitution and structural analysis, together with functional studies, are bringing to light the organizational principles of these complex and fascinating structures. We summarize recent work and identify a few challenges for future work.