Substrate oxidation by isolated rat brain mitochondria and synaptosomes.
Substrate oxidation by isolated rat brain mitochondria and synaptosomes.
复制标题
分离的大鼠脑线粒体和突触体的底物氧化。
DOI：
10.1002/jnr.490140206
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发表时间：
1985
影响因子：
4.2
通讯作者：
Stevenson,JH
中科院分区：
文献类型：
--
作者：
Tildon,JT;Roeder,LM;Stevenson,JH
胞浆和线粒体或胞浆和突触体重组系统对[6-14C]-葡萄糖的氧化速率与[3-14C]-3-羟基丁酸酯的氧化速率基本相同。然而，线粒体氧化[U-14C]-谷氨酰胺的速率是突触体氧化速率的2.5倍。加入谷氨酰胺(5 MM)后，线粒体和突触体氧化[6-14C]-葡萄糖的速率分别降低20%和40%。相反，添加葡萄糖(5 MM)对任一细胞器的[U-14C]-谷氨酰胺氧化速率几乎没有影响。氨基氧乙酸酯使线粒体氧化[U-14C]-谷氨酰胺的速率降低35%以上，但对突触体氧化谷氨酰胺的速率影响很小或没有影响。在[3-14C]-3-羟基丁酸酯中加入5 mM葡萄糖后，线粒体和突触体的氧化速率分别增加了65%和77%。然而，在相反的情况下，加入3-羟基丁酸酯可减少突触体对[6-14C]-葡萄糖的氧化(35%)，但不会降低线粒体的氧化速率。这些结果表明，线粒体和突触体对底物利用率的不同以及这两个亚细胞细胞器中底物相互作用的不同可能有助于脑内的代谢区划。
The rates of [6‐14C]‐glucose oxidation by reconstituted systems of cytosol and mitochondria or cytosol and synaptosomes were essentially the same as the rate of oxidation of [3‐14C]‐3‐hydroxybutyrate. However, the rate of [U‐14C]‐glutamine oxidation by mitochondria was 2.5 times that by synaptosomes. The addition of glutamine (5 mM) caused a reduction in the rates of oxidation of [6‐14C]‐glucose of 20% and 40% by mitochondria and synaptosomes, respectively. Conversely, the addition of glucose (5 mM) had little or no effect on the rate of [U‐14C]‐glutamine oxidation by either organelle. Amino‐oxyacetate decreased [U‐14C]‐glutamine oxidation by mitochondria more than 35% but had little or no effect on the rate of glutamine oxidation by synaptosomes. When glucose (5 mM) was added to [3‐14C]‐3‐hydroxybutyrate, the rates of oxidation by the mitochondria and synaptosomes were increased by 65% and 77%, respectively. However, in the reverse situation the addition of 3‐hydroxybutyrate decreased [6‐14C]‐glucose oxidation by synaptosomes (35%) but did not decrease the rate by mitochondria. These results suggest that differences in the rates of substrate utilization by mitochondria and synaptosomes and differences in substrate interactions in these two subcellular organelles may contribute to metabolic compartmentation in the brain.