Monoclonal antibodies SMI 311 and SMI 312 as tools to investigate the maturation of nerve cells and axonal patterns in human fetal brain
Monoclonal antibodies SMI 311 and SMI 312 as tools to investigate the maturation of nerve cells and axonal patterns in human fetal brain
复制标题
单克隆抗体 SMI 311 和 SMI 312 作为研究人胎儿大脑神经细胞成熟和轴突模式的工具
DOI：
10.1007/s004410051013
复制
发表时间：
1998
影响因子：
3.6
通讯作者：
J. Bohl
中科院分区：
文献类型：
--
作者：
N. Ulfig;J. Nickel;J. Bohl
摘要神经丝是神经系统成熟过程中最早被发现的特征之一，它只存在于神经细胞中。 神经丝蛋白在神经元内不同磷酸化程度的差异分布提供了选择性地显示胞体和树突或轴突的可能性。借助于单克隆抗体SMI 311可以观察到胞体和树突的典型的非磷酸化神经丝，而抗体SMI 312针对神经丝的高度磷酸化轴突表位。神经元类型的成熟，区域特异性轴突网络的发展，以及成熟的梯度，因此可以证明。当标本在多聚甲醛和苦味酸的混合物中固定长达3天，切片自由漂浮孵育时，SMI 311和SMI 312的免疫染色效果最佳。神经元及其树枝状结构域以类似Golgi的方式被SMI 311免疫染色，可以在相对较厚的切片中完全可视化。高尔基体制备的局限性，如胶质细胞标记，伪影，和染色只有一个小的非代表性的百分比现有的神经元，是不明显的SMI制剂，这另外提供了选择性染色轴突网络的可能性。在正常胎儿脑中所获得的结果为发育障碍的研究提供了基础。
Abstract Neurofilaments, which are exclusively found in nerve cells, are one of the earliest recognizable features of the maturing nervous system. The differential distribution of neurofilament proteins in varying degrees of phosphorylation within a neuron provides the possibility of selectively demonstrating either somata and dendrites or axons. Non-phosphorylated neurofilaments typical of somata and dendrites can be visualized with the aid of monoclonal antibody SMI 311, whereas antibody SMI 312 is directed against highly phosphorylated axonal epitopes of neurofilaments. The maturation of neuronal types, the development of area-specific axonal networks, and the gradients of maturation can thus be demonstrated. Optimal immunostaining with SMI 311 and SMI 312 is achieved when specimens are fixed in a mixture of paraformaldehyde and picric acid for up to 3 days and sections are incubated free-floating. Neurons, with their dendritic domains immunostained by SMI 311 in a Golgi-like manner, can be completely visualized in relatively thick sections. The limitations of Golgi-preparations, such as glia-labeling, artifacts, and the staining of only a small non-representative percentage of existing neurons, are not apparent in SMI preparations, which additionally provide the possibility of selectively staining axonal networks. The results achieved in normal fetal brain provide the basis for studies of developmental disturbances.