In vitro and in vivo hemocompatibility assessment of ultrathin sulfobetaine polymer coatings for silicon-based implants
In vitro and in vivo hemocompatibility assessment of ultrathin sulfobetaine polymer coatings for silicon-based implants
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DOI：
10.1177/0885328219831044
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发表时间：
2019-08-01
影响因子：
2.9
通讯作者：
Roy, Shuvo
中科院分区：
文献类型：
--
作者：
Iqbal, Zohora;Kim, Steven;Roy, Shuvo
高度均匀的硅纳米孔膜被开发用于可植入的生物人工器官。需要一种坚固的、易于扩展的、无污染的表面涂层来增强硅纳米孔膜的血液相容性。然而，涂层必须是多孔的，以防止纳米孔堵塞。最近，两性离子刷状聚合物已经证明在生物条件下显著更低的结垢。在这项研究中，我们探讨了两性离子聚（磺基甜菜碱甲基丙烯酸酯）（pSBMA）的表面涂层在5 nm以下的厚度。在硅纳米孔膜的表面改性之前和之后测量膜的水力渗透性，并且发现孔是专利的并且与涂层厚度测量一致。在生物剪切下以及在体外和体内血流下分析涂层稳定性。暴露于剪切超过24小时后，通过X射线光电子能谱、测角术和椭圆偏振仪对涂层进行表征，发现涂层能够承受生物剪切。使用新鲜人血的体外血液实验以及猪模型中的体内7天和26天植入物证明，与暴露于体外新鲜人血的未改性硅相比，pSBMA表面改性的血小板粘附和活化最小。这些结果表明，在具有关键纳米级特征的血液接触植入物中应用，PIPSBMA表面改性是一种可行的选择。
Highly uniform silicon nanopore membranes were developed for applications in implantable bioartificial organs. A robust, readily scalable, non-fouling surface coating is required to enhance silicon nanopore membrane hemocompatibility. However, the coating must be ultrathin to keep the nanopores from occluding. Recently, zwitterionic brush polymers have demonstrated significantly lower fouling under biological conditions. In this study, we explore ultrathin zwitterionic poly(sulfobetaine methacrylate) (pSBMA) surface coating at sub-5 nm thickness. Membrane hydraulic permeability was measured before and after surface modification of silicon nanopore membranes, and pores were found to be patent and in agreement with coating thickness measurements. Coating stability was analyzed under biological shear as well as under blood flow in vitro and in vivo. Following exposure to shear over 24 h, coatings were characterized via X-ray photoelectron spectroscopy, goniometry, and ellipsometry, and found to survive biological shear. In vitro blood experiments with fresh human blood as well as in vivo 7-day and 26-day implants in a porcine model demonstrate minimal platelet adhesion and activation with pSBMA surface modification compared to unmodified silicon exposed to fresh human blood in vitro. These results demonstrate that ultrathin pSBMA surface modification is a viable choice for application in blood contacting implants with critical nanoscale features.