Expression of HLA-DR on T lymphocytes following renal transplantation, and association with graft-rejection episodes and cytomegalovirus infection.
Expression of HLA-DR on T lymphocytes following renal transplantation, and association with graft-rejection episodes and cytomegalovirus infection.
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肾移植后 T 淋巴细胞上 HLA-DR 的表达，以及与移植物排斥事件和巨细胞病毒感染的关系。
DOI：
10.1097/00007890-198401000-00018
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发表时间：
1984
期刊：
影响因子：
6.2
通讯作者：
L. A. V. Es
中科院分区：
文献类型：
--
作者：
A. V. Es;William M. Baldwin;P. Oljans;H. Tanke;J. Ploem;L. A. V. Es
通过单克隆抗体和流式细胞术识别的 T 淋巴细胞亚群的 HLA-DR 表达在 10 名正常对照、15 名血液透析患者、25 名移植功能稳定的同种异体肾移植受者、16 名遭受排斥反应的移植受者和 4 名巨细胞病毒 (CMV)4 感染的移植受者中进行了监测。在这些对照和患者的 OKT4 反应亚群中，没有观察到 HLA-DR 表达的显着差异。在 16 名遭受排斥反应的患者中，观察到 OKT8 反应细胞的 HLA-DR 表达显着增加。在移植后 1 周后发生排斥反应的 10 名患者中，表达 HLA-DR 的 OKT8 反应细胞的百分比在排斥反应临床症状出现前 2-6 天显着增加（P 小于 0.001 Student's t 检验）。当排斥反应对治疗有反应时，OKT8 反应细胞上的 HLA-DR 表达下降，而在 3 名因不可逆排斥而失去移植物的患者中，HLA-DR 表达仍然升高。此外，4 名 CMV 感染患者的表达 HLA-DR 的 OKT8 反应细胞百分比增加（P 小于 0.001 Student's t 检验）。 HLA-DR表达的增加与CMV感染的临床症状的出现同时发生，并且在CMV抗体滴度显着增加且感染症状消失后，HLA-DR的表达恢复到正常值。
The expression of HLA-DR by T lymphocyte subpopulations recognized by monoclonal antibodies and flow cytometry was monitored in 10 normal controls, 15 patients on hemodialysis, 25 recipients of a renal allograft with stable graft function, 16 transplant recipients suffering rejection episodes, and 4 transplant recipients with cytomegalovirus (CMV)4 infection. No significant differences in HLA-DR expression were observed in the OKT4-reactive subpopulation among these controls and patients. In the 16 patients who suffered rejection episodes a significant increase in HLA-DR expression by OKT8-reactive cells was observed. In the 10 patients in whom rejection episodes occurred later than 1 week after transplantation, the percentage of OKT8-reactive cells that expressed HLA-DR was dramatically increased 2-6 days before the onset of clinical symptoms of rejection (P less than 0.001 Student's t test). When the rejection episode responded to treatment, expression of HLA-DR on the OKT8-reactive cells decreased--whereas in 3 patients who lost their grafts because of irreversible rejection, expression of HLA-DR remained elevated. In addition, the 4 patients with CMV infection had increased percentages of OKT8-reactive cells that expressed HLA-DR (P less than 0.001 Student's t test). This increase in expression of HLA-DR coincided with the onset of clinical symptoms of the CMV infection, and the expression of HLA-DR returned to normal values after the titers of antibodies to CMV had increased significantly and symptoms of infection had disappeared.