Reduced uterine perfusion pressure model is not successful to mimic severe preeclampsia
Reduced uterine perfusion pressure model is not successful to mimic severe preeclampsia
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DOI：
10.1016/j.placenta.2011.06.005
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发表时间：
2011-09-01
期刊：
影响因子：
3.8
通讯作者：
Cetin, M.
中科院分区：
文献类型：
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作者：
Balta, O.;Boztosun, A.;Cetin, M.
本研究旨在探讨先兆子痫大鼠模型的母体参数(血红蛋白、红细胞压积、血和尿液生化指标)和胎儿参数(活胎数和体重)。同时检测胎盘氧化应激标志物(蛋白质羰基、丙二醛)和胎盘抗氧化值(铜锌超氧化物歧化酶、谷胱甘肽过氧化物酶)。采用降低子宫灌流压(RUPP)模型，建立了定时妊娠SD大鼠先兆子痫模型。胎盘氧化应激在胎盘相关疾病的病理生理学中起关键作用，尤其是子痫前期(PE)和宫内生长受限(IUGR)。在妊娠第14天，将银夹放置在肾动脉下方的主动脉周围和卵巢动脉的左右子宫拱部。在RUPP模型动物(n=15)中，与正常血压对照组(n=15)相比，RUPP大鼠妊娠第19天的动脉压(151.7±17.6 mm Hg)显著高于正常妊娠大鼠(113.9±11.4 mm Hg)。与正常妊娠大鼠相比，RUPP大鼠蛋白质排泄量显著增加(0.3+/-0.04vs0.47+/-0.07g/dL)(p<；0.05)。与高血压相关的是，胎盘组织中丙二醛(2.4+/-0.2比1.6+/-0.2 umol/g组织)和蛋白质羰基(1.4+/-0.3比0.9+/-0.3nmol/mg蛋白质)水平升高，而超氧化物歧化酶(0.03比0.42U/mg蛋白质)和谷胱甘肽过氧化物酶(1.04+/-0.31比0.76+/-0.22U/g蛋白质)水平降低。子痫前期组的仔数(6.6+/-3.1对9.93+/-2.0)和窝重(17.4+/-7.7对22.9+/-6.7克)较低。除钠、氯外，子痫前期组全血细胞计数和生化指标均无显著差异。我们的发现表明，RUPP模型不能模拟重度子痫前期；然而，使用不同环境的进一步研究可能有助于获得能够成功产生严重子痫前期结果的先兆子痫模型。皇冠版权所有(C)2011由爱思唯尔有限公司出版。保留所有权利。
The aim of the study was to investigate maternal (hemoglobin, hematocrit, and biochemical parameters of blood and urine) and fetal parameters (number and weight of alive fetus) of preeclampsia in a rat model. Placental oxidative stress markers (protein carbonyl, malondialdehyde) and placental antioxidant values (CuZn-superoxide dismutase, glutathione peroxidase) were also measured. Preeclampsia was induced experimentally in timed-pregnant Sprague-Dawley rats by using the reduced uterine perfusion pressure (RUPP) model. Placental oxidative stress that plays a key role in the pathophysiology of placenta-related disorders, most notably preeclampsia (PE) and intrauterine growth restriction (IUGR) were demonstrated by using RUPP model. On day 14 of gestation, silver clips were placed around the aorta below the renal arteries and on the left and right uterine arcade at the ovarian artery. In the RUPP model animals (n = 15), when compared with the normotensive controls (n = 15), arterial pressure on day 19 of gestation was significantly higher in the RUPP rats (151.7 +/- 17.6 mmHg) than normal pregnant rats (113.9 +/- 11.4 mmHg). The RUPP rats showed a significant increase in protein excretion when compared with the normal pregnant rats (0.3 +/- 0.04 vs 0.47 +/- 0.07 g/dL) (p < 0.05). Associated with the hypertension in RUPP rats, placental levels of malondialdehyde (2.4 +/- 0.2 vs. 1.6 +/- 0.2 umol/gm tissue) and protein carbonyl (1.4 +/- 0.3 vs. 0.9 +/- 0.3 nmol/mg protein) were increased, while superoxid dismutase (0.03 vs 0.42 U/mg protein) and glutathione peroxidase (1.04 +/- 0.31 vs 0.76 +/- 0.22 U/g protein) were decreased. Pup number (6.6 +/- 3.1 vs. 9.93 +/- 2.0) and litter weight (17.4 +/- 7.7 vs. 22.9 +/- 6.7 g) were lower in the preeclamptic group. None of the complete blood counts and biochemical values other than sodium and chlorine were significantly different in preeclamptic group. Our findings suggest that the RUPP model cannot mimic severe preeclampsia; however, further studies using different settings may be helpful to obtain a preeclampsia model that is capable of succesfully producing severe preeclampsia findings. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.