CD155 expression and its correlation with clinicopathologic characteristics, angiogenesis, and prognosis in human cholangiocarcinoma.
CD155 expression and its correlation with clinicopathologic characteristics, angiogenesis, and prognosis in human cholangiocarcinoma.
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CD155在人胆管癌中的表达及其与临床病理特征、血管生成和预后的相关性
DOI：
10.2147/ott.s141476
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发表时间：
2017
影响因子：
4
通讯作者：
Huang Q
中科院分区：
文献类型：
--
作者：
Huang DW;Huang M;Lin XS;Huang Q
背景CD155是一种免疫球蛋白样黏附分子，在肿瘤细胞的迁移、增殖、转移和肿瘤免疫等过程中发挥重要作用。CD155在多种人类恶性肿瘤中表达上调，但其在胆管细胞癌(CCA)中的表达仍不清楚。本研究旨在探讨CD155在CCA中的表达及其与临床病理资料、血管生成和预后的关系。材料与方法采用Western blotting和实时荧光定量聚合酶链式反应检测CD155在20例配对CCA组织和相应癌旁组织中的蛋白和mRNA表达。此外，本研究还通过免疫组织化学方法检测了90例CCA中CD155的表达与血管内皮生长因子和肿瘤内微血管密度的关系。并用免疫组织化学方法评价CD155在CCA中的临床和预后意义。结果CCA瘤组织中CD155的蛋白和mRNA水平均高于相应的癌旁组织(P<；0.05)。免疫组织化学染色显示CD155定位于癌细胞胞浆，在61.2%(55/90)的CCA组织中高表达。CD155的表达水平与肿瘤组织学分级(P=0.002)、淋巴结转移(P<；0.001)和肿瘤转移(P=0.03)显著相关。Spearman等级相关分析显示，CD155的表达与血管内皮生长因子(r=0.481，P<0.001)和微血管密度(r=0.442，P<0.001)呈正相关。更重要的是，CD155高表达患者术后总生存期(P<；0.001)和无瘤生存期(P<；0.001)显著缩短，多因素分析显示CD155高表达是影响总生存期和无瘤生存期的独立预后因素(P<；0.001)。结论CD155表达上调与CCA侵袭性临床病理特征、血管生成及预后不良有关，可作为CCA患者预后的生物标志物。
Background CD155, an immunoglobulin-like adhesion molecule, plays an important role in carcinoma such as cells migration, proliferation, metastasis, and tumor immune. The upregulation of CD155 has been found in several human malignancies, but its expression in cholangiocarcinoma (CCA) still remains unclear. The aim of this study is to investigate CD155 expression and its correlations with clinicopathologic data, angiogenesis, and prognosis in the patients with CCA. Materials and methods CD155 expression was investigated in 20 paired CCA tissues and corresponding paracancerous tissues by Western blotting and quantitative real-time polymerase chain reaction assays at protein and mRNA levels. Besides, this study evaluated the correlation between the tumor CD155 expression and the level of both vascular endothelial growth factor and intratumoral microvessel density by immunohistochemistry in 90 cases of CCA. Moreover, the clinical and prognostic significance of CD155 in CCA was assessed by immunohistochemistry. Results The protein and mRNA levels of CD155 were higher in CCA tumor tissues compared with corresponding paracancerous tissues (P<0.05). Immunohistochemical staining showed that CD155 was located in the cytoplasm of carcinoma cells and overexpressed in 61.2% (55/90) CCA tissues. Obviously, CD155 expression level was significantly correlated with tumor histological grade (P=0.002), lymph node metastasis (P<0.001), and tumor-node-metastasis (P=0.03). Additionally, Spearman rank correlation test demonstrated that CD155 expression was positively associated with vascular endothelial growth factor (r=0.481, P<0.001) and microvessel density (r=0.442, P<0.001) in CCA tissues. More importantly, CCA patients with high CD155 expression had a markedly shorter overall survival (P<0.001) and disease-free survival (P<0.001) after surgical resection, and multivariate analysis showed that high CD155 expression was an independent poor prognostic predictor of overall survival and disease-free survival (P<0.001). Conclusion Our results revealed that upregulated CD155 correlated with aggressive clinicopathologic characteristics, angiogenesis, and poor prognosis in CCA and may be a promising prognostic biomarker for the CCA patients.