Analysis of propolis: some parameters and procedures for chemical quality control
Analysis of propolis: some parameters and procedures for chemical quality control
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DOI：
10.1080/00218839.1998.11100961
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发表时间：
1998-01-01
影响因子：
1.9
通讯作者：
Salatino, A
中科院分区：
文献类型：
--
作者：
Woisky, RG;Salatino, A
一组参数和相应的程序建立的化学配置文件的样品的酊剂和原油的蒸馏水。建议将总酚、黄酮、蜡、灰分、挥发性物质和干残渣的含量估计值作为表征样品的参数。对于酊剂，提出了总酚类物质、类黄酮、蜡、比重和乙醇的估计。总酚类物质和黄酮类化合物测定分光光度法，蜡重量和乙醇气相色谱法。的准确度的分光光度法程序进行了测试，通过测定一个已知的酚酸和黄酮类化合物的混合物。使用的程序是举例说明了6个样品的粗品的分析，从不同的地方在巴西和酊剂制备的绝对和70%的乙醇水溶液。总酚类物质，黄酮类化合物，蜡和挥发性化合物的分析样品的含量相对较低，与文献中的数据相比。来自附近地区的样品可能具有完全不同的化学特征。与无水乙醇相比，用含水乙醇提取导致无蜡酊剂，含有更高量的酚类物质。
A set of parameters and respective procedures for the establishment of chemical profiles of samples of tinctures and crude propolis is presented. It is proposed that estimations of the content of total phenolic substances, flavonoids, waxes, ash, volatile substances and dry residue be used as parameters to characterize samples of crude propolis. For tinctures, the estimations of total phenolic substances, flavonoids, waxes, specific gravity and ethanol are proposed. Total phenolic substances and flavonoids are measured by spectrophotometric methods, waxes gravimetrically and ethanol by gas chromatography. The accuracy of the spectrophotometric procedures was tested by assaying a mixture with a known composition of phenolic acids and flavonoids. The use of the procedures is exemplified by the analyses of six samples of crude propolis from different localities in Brazil and of tinctures prepared with absolute and 70% aqueous ethanol. The contents of total phenolic substances, flavonoids, waxes and volatile compounds of the samples analysed are relatively low in comparison with data from the literature. Samples of propolis from nearby localities may have quite different chemical profiles. Compared with absolute ethanol, extraction with aqueous ethanol results in wax-free tinctures, containing higher amounts of phenolic substances.