Giving structure to the biofilm matrix: an overview of individual strategies and emerging common themes.
Giving structure to the biofilm matrix: an overview of individual strategies and emerging common themes.
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DOI：
10.1093/femsre/fuv015
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发表时间：
2015-09
影响因子：
11.3
通讯作者：
Stanley-Wall NR
中科院分区：
文献类型：
--
作者：
Hobley L;Harkins C;MacPhee CE;Stanley-Wall NR
生物膜是微生物细胞的群落，其支撑多种过程，包括污水生物修复、植物生长促进、慢性感染和工业生物污损。驻留在生物膜中的细胞被包裹在通常包含脂质、经常表现出淀粉样性质的蛋白质、eDNA和胞外多糖的自产外聚物基质内。这种基质为群落履行各种功能，从提供结构刚性和保护免受外部环境影响到控制基因调控和营养吸收。开发控制生物膜感染的新策略或利用生物膜形成的力量用于工业和生物技术用途的能力的关键是对生物膜基质的深入了解。这与单个组分的结构、分子之间相互作用的性质以及三维空间组织有关。我们强调最近的进展，在理解的结构和功能的作用，碳水化合物和蛋白质在生物膜基质内发挥提供三维建筑的完整性和功能的生物膜社区。我们强调，在相关的实验技术，使我们的理解的生物膜基质的边界扩展使用大肠杆菌，金黄色葡萄球菌，霍乱弧菌，枯草芽孢杆菌作为范例。检查生物膜细胞外基质的结构和功能。检查生物膜细胞外基质的结构和功能。
Biofilms are communities of microbial cells that underpin diverse processes including sewage bioremediation, plant growth promotion, chronic infections and industrial biofouling. The cells resident in the biofilm are encased within a self-produced exopolymeric matrix that commonly comprises lipids, proteins that frequently exhibit amyloid-like properties, eDNA and exopolysaccharides. This matrix fulfils a variety of functions for the community, from providing structural rigidity and protection from the external environment to controlling gene regulation and nutrient adsorption. Critical to the development of novel strategies to control biofilm infections, or the capability to capitalize on the power of biofilm formation for industrial and biotechnological uses, is an in-depth knowledge of the biofilm matrix. This is with respect to the structure of the individual components, the nature of the interactions between the molecules and the three-dimensional spatial organization. We highlight recent advances in the understanding of the structural and functional role that carbohydrates and proteins play within the biofilm matrix to provide three-dimensional architectural integrity and functionality to the biofilm community. We highlight, where relevant, experimental techniques that are allowing the boundaries of our understanding of the biofilm matrix to be extended using Escherichia coli, Staphylococcus aureus, Vibrio cholerae, and Bacillus subtilis as exemplars. Examining the structure and function of the biofilm extracellular matrix. Examining the structure and function of the biofilm extracellular matrix.