PFKFB3 was overexpressed in gastric cancer patients and promoted the proliferation and migration of gastric cancer cells
PFKFB3 was overexpressed in gastric cancer patients and promoted the proliferation and migration of gastric cancer cells
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PFKFB3在胃癌患者中过表达并促进胃癌细胞的增殖和迁移
DOI：
10.3233/cbm-160143
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发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Wu, Guohao
中科院分区：
文献类型：
--
作者：
Han, Jun;Meng, Qingyang;Wu, Guohao
目的：胃癌是世界范围内最常见的癌症之一，由于缺乏特异性和敏感的生物标志物，预后仍然很差。有氧糖酵解是胃癌细胞的重要标志之一，并且几种糖酵解酶在胃癌患者中高度表达。方法：采用qRT-PCR、免疫组化和western blot方法检测134例胃癌患者组织中PFKFB 3的表达，并分析PFKFB 3在胃癌组织中的表达与临床意义。分析PFKFB 3表达与临床病理因素的相关性。2测试。结果：PFKFB 3在81.3%（109/ 134）的胃癌组织中高表达，且在胃癌组织中的表达率明显高于对照组（P < 0.05）。PFKFB 3过表达与淋巴结转移（P = 0.045）和TNM分期（P = 0.033）有关。结论：PFKFB 3可能是一种潜在的胃癌生物标志物和抗肿瘤靶向基因。
OBJECTIVE: Gastric cancer is one of the most common cancers worldwide, and the prognosis is still very poor due to the lack of specific and sensitive biomarkers. Aerobic glycolysis is one of the critical hallmarks of gastric cancer cells, and several glycolytic enzymes are highly expressed in gastric cancer patients. However, the expression and clinical significances of phosphofructokinase-2/fructose-2,6-bisphosphatase3 (PFKFB3, one of the glycolytic enzymes) in a large sample of gastric cancer patients remain unclear.METHODS: The expression of PFKFB3 was detected in 134 gastric cancer patients by qRT-PCR, immunohistochemistry, and western blot analyses. The correlation between PFKFB3 expression and clinicopathological factors was analyzed by. 2 test. In addition, we also analyzed whether the knockdown of PFKFB3 by siRNAs could inhibit the ability of gastric cancer cells (MGC-803 and AGS) to proliferate and migrate by MTT analysis and transwell analyses.RESULTS: PFKFB3 was highly expressed in 81.3% (109/ 134) of gastric cancer patients. The overexpression of PFKFB3 was associated with lymph node metastasis (P = 0.045) and TNM stage (P = 0.033). Knockdown of PFKFB3 by siRNAs significantly inhibited the proliferation and migration abilities of gastric cancer cells.CONCLUSION: Our data suggest that PFKFB3 might be a potential biomarker for gastric cancer and anti-neoplastic targeting gene.