A tissue culture system for different germplasms of indica rice
A tissue culture system for different germplasms of indica rice
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DOI：
10.1007/s00299-005-0100-7
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发表时间：
2006-05-01
影响因子：
6.2
通讯作者：
Wang, SP
中科院分区：
文献类型：
--
作者：
Ge, XJ;Chu, ZH;Wang, SP
农杆菌介导的籼稻转化仅在有限的品种中进行，因为大多数籼稻品种对离体反应不敏感。建立高效、广泛应用的籼稻组织培养体系，将促进转化技术在育种中的应用和对籼稻特异基因功能的研究。通过对培养基中植物生长调节剂、有机成分和盐的调控，建立了籼稻愈伤组织诱导和继代培养的两种培养基。改良培养基能保证不同籼稻品种成熟种子胚性愈伤组织的大量产生和增殖。该体系所获得的愈伤组织是农杆菌介导转化的理想材料。结果表明，该优化的组织培养体系可广泛应用于籼稻品种的组织培养。
Agrobacterium-mediated transformation of indica rice has been manipulated in only a limited number of cultivars because the majority of indica varieties are recalcitrant to in vitro response. Establishment of a highly efficient and widely used tissue culture system for indica rice will accelerate the application of transformation technology in breeding programs and the study of the functions of indica-specific genes. By manipulating plant growth regulators, organic components and salts within the culture media, we established two media for callus induction and subculture, respectively, in tissue culture of indica rice. The modified media could guarantee the production and proliferation of a great number of embryogenic calli with high regeneration capacity from mature seeds representing different indica rice germplasms. The calli obtained from this system should be ideal material for Agrobacterium-mediated transformation. The results suggest that this optimized tissue culture system will be widely applicable for the tissue culture of indica varieties.