PtFCA from precocious trifoliate orange is regulated by alternative splicing and affects flowering time and root development in transgenic Arabidopsis
PtFCA from precocious trifoliate orange is regulated by alternative splicing and affects flowering time and root development in transgenic Arabidopsis
复制标题
来自早熟三叶橙的 PtFCA 通过选择性剪接进行调节，并影响转基因拟南芥的开花时间和根部发育
DOI：
10.1007/s11295-016-1035-6
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发表时间：
2016
影响因子：
2.4
通讯作者：
Hu Chun-Gen
中科院分区：
文献类型：
--
作者：
Ai Xiao-Yan;Zhang Jin-Zhi;Liu Tian-Jia;Hu Chun-Gen
向开花的转变是显花植物的一个主要发育转变。核RNA结合蛋白FCA响应季节信号和脱落酸（阿坝），可以通过环境温度和自主途径控制开花时间。从早熟的枳橙子（Poncirus trifoliata L.）中分离到柑橘FCA同源基因（PtFCA）。Raf）。分离出PtFCA的三种选择性剪接转录物（PtFCA 1、PtFCA 2和PtFCA 3）。PtFCA的表达模式表明PtFCA可能参与了早熟橙子的阶段转换。PtFCA的功能互补实验表明，PtFCA 1部分拯救了fca-1突变体的晚花表型。PtFCA 3对转基因拟南芥的开花时间没有影响，而PtFCA 2在fca-1背景下表现出延迟开花时间。同时，在fca-1背景下，这三种转录本对根的发育也表现出不同的调控能力。蛋白质-蛋白质相互作用的研究表明，PtFCA可能与PtFY和PtNF-YA 7形成高级复合物，调控早熟枳橙子从营养生长期向生殖生长期过渡的时间。阿坝和室温处理改变了PtFCA和互作蛋白的表达。这些结果表明，PtFCA可能通过形成多种蛋白复合物，对早熟橙子的开花时间和根系发育起重要作用。
The transition to flowering is a major developmental switch in flowering plants. The nuclear RNA-binding protein FCA responds to seasonal signals and abscisic acid (ABA), which can control the flowering time via ambient temperature and autonomous pathways. Citrus FCA ortholog (PtFCA) has been isolated and characterized from precocious trifoliate orange (Poncirus trifoliata L. Raf). Three alternatively spliced transcripts of PtFCA (PtFCA1, PtFCA2, and PtFCA3) were isolated. The expression pattern of PtFCA indicated that it may be involved in phase transition in precocious trifoliate orange. A functional complementation experiment of PtFCA indicated that PtFCA1 partially rescued the late-flowering phenotype of the fca-1 mutant. There was no influence on flowering time of transgenic Arabidopsis by PtFCA3 as compared with PtFCA2, which exhibits delayed flowering time in a fca-1 background. Meanwhile, these three transcripts also showed different abilities to regulate root development in the fca-1 background. The study of protein–protein interactions suggested that PtFCA may form higher order complexes with PtFY and PtNF-YA7 to regulate timing of the transition from the vegetative to reproductive phase in precocious trifoliate orange. ABA and ambient temperature treatments changed the expression of PtFCA and interaction protein. These findings reveal that PtFCA may play important roles in flowering time and root development of precocious trifoliate orange through the formation of multiple protein complexes.