UNIDIRECTIONAL BUDDING OF HIV-1 AT THE SITE OF CELL-TO-CELL CONTACT IS ASSOCIATED WITH CO-POLARIZATION OF INTERCELLULAR-ADHESION MOLECULES AND HIV-1 VIRAL MATRIX PROTEIN
UNIDIRECTIONAL BUDDING OF HIV-1 AT THE SITE OF CELL-TO-CELL CONTACT IS ASSOCIATED WITH CO-POLARIZATION OF INTERCELLULAR-ADHESION MOLECULES AND HIV-1 VIRAL MATRIX PROTEIN
复制标题
DOI：
10.1097/00002030-199509040-00003
复制
发表时间：
1995-04-01
期刊：
影响因子：
3.8
通讯作者：
DIANZANI, F
中科院分区：
文献类型：
--
作者：
FAIS, S;CAPOBIANCHI, MR;DIANZANI, F
目的：探讨HIV-1出芽和细胞粘附分子在细胞接触部位共同表达的可能性。目的：研究宿主细胞来源的粘附分子在HIV-1中的掺入情况。方法：应用透射电镜观察HIV-1出芽过程中的细胞部位。免疫细胞化学单、双染色检测病毒基质蛋白和粘附分子在细胞内的分布。流式细胞术定量检测细胞粘附分子的表达。免疫捕获技术用于测量HIV-1上细胞衍生蛋白的存在。通过p24抗原测定来测量捕获的病毒。结果：释放和出芽的HIV-1主要定位于细胞与细胞接触区。该特征与细胞间接触区域的病毒基质蛋白p18的极化染色一致。HIV-1感染细胞中的细胞间粘附分子（ICAM）-1在分离的细胞和合胞体上都被极化，与HIV-1基质蛋白共定位。HIV-1纳入所有的粘附分子表达的宿主细胞，虽然没有定量相关性与他们的细胞expressions.Conclusions：HIV-1被释放在细胞与细胞膜接触网站。ICAM-1和病毒基质蛋白在未感染细胞的募集所涉及的位点上在分离的细胞和合胞体上共极化。在大多数病毒体被释放的细胞部位，ICAM的浓度令人印象深刻，这可能是HIV-1后代获得这些膜蛋白的原因，并且可能对细胞介导的传播很重要。
Objectives: To explore the possibility that HIV-1 budding and cellular adhesion molecules co-polarize at cell-to-cell contact sites. To investigate the incorporation of host-cell-derived adhesion molecules into HIV-1.Methods: The cellular sites involved in HIV-1 budding were examined by transmission electron microscopy. Single and double immunocytochemistry staining was used to evaluate the cellular distribution of the viral matrix protein and adhesion molecules. Quantitative flow cytometry was used to measure the cellular expression of adhesion molecules. An immunocapture technique was used to measure the presence of cell-derived proteins on HIV-1. The captured virus was measured by a p24 antigen assay. The infectivity of virus captured by monoclonal antibodies was tested by measuring the virus antigen yield in supernatants after the addition of sensitive cells.Results: Released and budding HIV-1 was mainly localized at the cell-to-cell contact regions. This feature was consistent with a polarized staining for the virus matrix protein p18 at cell-to-cell contact regions. Intercellular adhesion molecules (ICAM)-1 in HIV-1-infected cells were polarized on both isolated cells and syncytia, co-localizing with HIV-1 matrix protein. HIV-1 incorporated all the adhesion molecules expressed by the host cells, although without quantitative correlation with their cellular expression.Conclusions: HIV-1 is released at cell-to-cell membrane contact sites. Both ICAM-1 and virus matrix protein co-polarized on isolated cells and syncytia at the sites involved in the recruitment of uninfected cells. The impressive concentration of ICAM at cell sites where most virions are released may account for the acquisition of these membrane proteins by the HIV-1 progeny, and may be important for the cell-mediated spread.