The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs
The Microbial Spectrum of Neonatal Sepsis in Uganda: Recovery of Culturable Bacteria in Mother-Infant Pairs
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DOI：
10.1371/journal.pone.0072775
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发表时间：
2013-08-27
期刊：
影响因子：
3.7
通讯作者：
Schiff, Steven J.
中科院分区：
文献类型：
--
作者：
Kiwanuka, Julius;Bazira, Joel;Schiff, Steven J.
发展中国家的新生儿败血症的特征还不完全。我们试图通过比较可从脓毒症新生儿及其母亲中培养的微生物来描述脓毒症中涉及的微生物谱，并确定母体传播的作用。我们从 80 对连续符合新生儿败血症临床标准的母婴中收集了婴儿血液和脊髓液以及母体血液和阴道标本。从 32.5% 婴儿的血液和 2.5% 的脑脊液培养物中回收到可识别的细菌，总共 35% 的可回收推定病原体。从阴道标本中回收的细菌与从婴儿中回收的细菌不一致。同样，从血液和脑脊液中回收的细菌也没有一致性。我们的结论是，依靠传统的细菌培养技术并不能充分描述这种情况下新生儿败血症的母体与环境来源的作用。需要更灵敏的分子方法来正确表征此类发展中国家新生儿败血症所涉及的母体和环境微生物群落。
Neonatal sepsis in the developing world is incompletely characterized. We seek to characterize the microbial spectrum involved in sepsis and determine the role of maternal transmission by comparing organisms that can be cultured from septic newborn infants and their mothers. From 80 consecutive mother-infant pairs meeting clinical criteria for neonatal sepsis, we collected infant blood and spinal fluid, and maternal blood and vaginal specimens. Identifiable bacteria were recovered from the blood in 32.5% of infants, and from 2.5% of cerebrospinal fluid cultures, for a total of 35% recoverable putative causative agents. Bacteria recovered from vaginal specimens were not concordant with those recovered from infants. Similarly there was no concordance of bacteria recovered from blood and cerebrospinal fluid. We conclude that relying on traditional bacterial culture techniques does not adequately delineate the role of maternal versus environmental sources of neonatal sepsis in this setting. More sensitive molecular approaches will be needed to properly characterize the maternal and environmental microbial community involved in neonatal sepsis in such developing countries.