The toxic effects of polystyrene microplastics on freshwater algae Chlorella pyrenoidosa depends on the different size of polystyrene microplastics
The toxic effects of polystyrene microplastics on freshwater algae Chlorella pyrenoidosa depends on the different size of polystyrene microplastics
复制标题
聚苯乙烯微塑料对淡水藻类蛋白核小球藻的毒性作用取决于聚苯乙烯微塑料的不同尺寸
DOI：
10.1016/j.chemosphere.2022.136135
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发表时间：
2022-09-02
期刊：
影响因子：
8.8
通讯作者：
Yang, Hui
中科院分区：
文献类型：
--
作者：
Cao, Qingsheng;Sun, Wenbo;Yang, Hui
微塑料(MPS)是持久性环境污染物。MPS对水生生物的毒性效应日益引起人们的关注，但其对水生浮游植物的毒性效应还没有得到深入的研究。研究了2、10、50 mg/L 2、10、50 mg/mMPS对蛋白核小球藻1、5、10d的毒性作用。测定其生长率、光合色素含量、胞外聚合物含量、可溶性蛋白含量、丙二醛含量以及光合作用和能量代谢相关基因的相对表达。结果表明，1MU/mMP对蛋白核拟青霉的生长有明显的抑制作用。与对照组相比，1 MU M MP显著降低了蛋白核小球藻的光合色素含量，诱导了氧化胁迫，破坏了细胞膜的完整性。在分子水平上，1MMMP改变了与光合作用和能量代谢相关的基因的转录水平。扫描电子显微镜和荧光图像显示，MPS与蛋白核拟青霉的聚集可能是MPS毒性作用的主要原因。这些结果将对不同MPS对水生浮游植物的毒性提供新的见解，并对MPS在水环境中造成的风险进行评估。
Microplastics (MPs) are persistent environmental contaminants. The toxic effects of MPs on aquatic organisms have raised increasing concerns, but their toxic effects on aquatic phytoplankton has not been thoroughly investigated. In the present study, the toxic effects of two sizes MPs (1 mu m and 5 mu m) on Chlorella pyrenoidosa at 2, 10, 50 mg/L were explored for 1, 5, 10 days. The growth ratio, photosynthetic pigments content, extracellular polymeric substances content, soluble protein content, MDA content and relative expression of genes related to photosynthesis and energy metabolism were measured. These results indicated that 1 mu m MP could significantly inhibit the growth of C. pyrenoidosa. Compared with the control group, 1 mu m MP significantly reduced the photosynthetic pigment content, induced oxidative stress and disrupted the cell membrane integrity of C. pyrenoidosa. At the molecular level, 1 mu m MP altered the transcript levels of genes related to photosynthesis and energy metabolism. Scanning electron microscopy and fluorescent images showed that MPs aggregation with C. pyrenoidosa may be the main reason for the toxic effects of MPs. These results will provide new insight into the toxicity of different MPs on aquatic phytoplankton, and evaluate the risks caused by MPs in aquatic environments.