The myostatin‐induced E3 ubiquitin ligase RNF13 negatively regulates the proliferation of chicken myoblasts
The myostatin‐induced E3 ubiquitin ligase RNF13 negatively regulates the proliferation of chicken myoblasts
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DOI：
10.1111/j.1742-4658.2009.07498.x
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发表时间：
2010-01
期刊：
影响因子：
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通讯作者：
Qiang Zhang;Kun Wang;Yong Zhang;Jiao Meng;Fangyuan Yu;Yan Chen;Dahai Zhu
中科院分区：
文献类型：
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作者：
Qiang Zhang;Kun Wang;Yong Zhang;Jiao Meng;Fangyuan Yu;Yan Chen;Dahai Zhu
泛素连接酶环指蛋白 13 基因 (RNF13) 首次在鸡胎儿成肌细胞中筛选其表达受肌生长抑制素调节的基因中被鉴定。在这项研究中，我们证明 RNF13 基因在许多鸡组织中广泛表达。 RNF13 的表达在骨骼肌发生过程中逐渐降低，肌肉生长抑制素在转录和翻译水平上调 RNF13 的表达。有趣的是，RNF13 的异位表达会抑制细胞增殖并抑制肌肉细胞中生肌基因 MyoD 和 Caveolin-3 的表达。此外，最近我们报道了RNF13是一种RING型E3泛素连接酶。在本报告中，我们提供的实验证据表明，破坏RING指的突变消除了RNF13的生长抑制活性，表明其E3连接酶活性是抑制细胞增殖所必需的。综上所述，我们的研究结果表明，RNF13 作为 E3 泛素连接酶发挥负向调节细胞增殖的作用。
The ubiquitin ligase RING finger protein 13 gene (RNF13) was first identified in a screen for genes whose expression is regulated by myostatin in chicken fetal myoblasts. In this study, we demonstrate that the RNF13 gene is broadly expressed in many chicken tissues. The expression of RNF13 gradually decreases during skeletal myogenesis, and myostatin up‐regulates RNF13 expression at both the transcriptional and translational levels. Interestingly, ectopic expression of RNF13 inhibits cell proliferation and suppresses the expression of the myogenic genes MyoD and Caveolin‐3 in muscle cells. Moreover, recently, we have reported that RNF13 is a RING‐type E3 ubiquitin ligase. In this report, we provide experimental evidence to show that mutations disrupting the RING finger abolish the growth‐suppressive activity of RNF13, indicating that its E3 ligase activity is required for the inhibition of cell proliferation. Taken together, our findings show that RNF13 functions as an E3 ubiquitin ligase to negatively regulate cell proliferation.