Atlas of Subcellular RNA Localization Revealed by APEX-Seq
Atlas of Subcellular RNA Localization Revealed by APEX-Seq
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DOI：
10.1101/454470
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发表时间：
2018-10
期刊：
影响因子：
64.5
通讯作者：
F. Fazal;Shuo Han;Pornchai Kaewsapsak;Kevin R. Parker;Jin Xu;A. Boettiger;Howard Y. Chang;A. Ting
中科院分区：
文献类型：
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作者：
F. Fazal;Shuo Han;Pornchai Kaewsapsak;Kevin R. Parker;Jin Xu;A. Boettiger;Howard Y. Chang;A. Ting
我们介绍APEX-seq，一种基于过氧化物酶APEX 2的空间邻近性的RNA测序方法。APEX-seq在9个不同的亚细胞区域产生了人类转录组的纳米分辨率空间图，揭示了不同RNA类别和转录异构体的广泛而精致的定位模式。我们发现了核转录组的放射状组织，其在核孔的内表面被门控，用于加工转录物的细胞质出口。我们确定了两种不同的信使RNA定位到线粒体的途径，每一种都与特定的转录本集相关，用于在细胞器内构建互补的大分子机器。APEX-seq应该广泛适用于许多系统，使空间转录组的全面调查。
We introduce APEX-seq, a method for RNA sequencing based on spatial proximity to the peroxidase enzyme APEX2. APEX-seq in nine distinct subcellular locales produced a nanometer-resolution spatial map of the human transcriptome, revealing extensive and exquisite patterns of localization for diverse RNA classes and transcript isoforms. We uncover a radial organization of the nuclear transcriptome, which is gated at the inner surface of the nuclear pore for cytoplasmic export of processed transcripts. We identify two distinct pathways of messenger RNA localization to mitochondria, each associated with specific sets of transcripts for building complementary macromolecular machines within the organelle. APEX-seq should be widely applicable to many systems, enabling comprehensive investigations of the spatial transcriptome.