Microbial electrosynthesis of acetate from CO2 in three-chamber cells with gas diffusion biocathode under moderate saline conditions
Microbial electrosynthesis of acetate from CO2 in three-chamber cells with gas diffusion biocathode under moderate saline conditions
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DOI：
10.1016/j.ese.2023.100261
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发表时间：
2023-04-07
影响因子：
12.6
通讯作者：
Farras, Pau
中科院分区：
文献类型：
--
作者：
Dessi, Paolo;Buenano-Vargas, Claribel;Farras, Pau
低电解液电导率和低效电池设计导致的高过电位阻碍了微生物电合成(MES)的工业应用。在这项研究中，来自于在盐水条件下运行的厌氧消化器(类似于13g L-1NaC l)的混合微生物群适合于在恒流(0.25 mA cm-2)H型槽中，在5，10，15或20g L-1NaC l浓度下从重碳酸盐中生产醋酸盐。仅在5g和10g L-1NaC l时，产乙酸菌群才被成功地富集，其抑制阈值约为6g L-1Na+。然后，这些富含浮游生物的群落被用作3D打印的三室细胞的接种物，这些细胞配备了气体扩散生物电极。电池充入CO2气体，恒流工作(0.25或1.00 mA·cm~(-2))。在5 g L浓度和1 mA cm(-2)电流下，乙酸乙酯的平均产率为44.7kgMWh(-1)，产率为55.4gm(-2)d(-1)(0.89g L-1d(-1))，库仑效率为82.4%。扫描电子显微镜和16S rRNA测序分析证实了以醋酸杆菌为主的阴极生物膜的形成。最后，将三个3D打印单元液压串联以模拟MES堆栈，在0.25 mA cm(-2)的条件下实现了三倍于单个单元的生产率。这证实了三室MES电池是一种高效且可扩展的二氧化碳生物电循环制醋酸酯的技术，适度的盐水条件(5g L-1NaC l)可以帮助降低他们的电力需求，同时保持乙酸菌的活性。(C)2023作者。爱思唯尔公司代表中国环境科学学会、哈尔滨工业大学、中国环境科学研究院出版。这是CC BY-NC-ND License(http://creativecommons.org/licenses/by-nc-nd/4.0/).下的一篇开放获取文章
The industrial adoption of microbial electrosynthesis (MES) is hindered by high overpotentials deriving from low electrolyte conductivity and inefficient cell designs. In this study, a mixed microbial consortium originating from an anaerobic digester operated under saline conditions (similar to 13 g L-1 NaCl) was adapted for acetate production from bicarbonate in galvanostatic (0.25 mA cm-2) H-type cells at 5,10,15, or 20 g L-1 NaCl concentration. The acetogenic communities were successfully enriched only at 5 and 10 g L-1 NaCl, revealing an inhibitory threshold of about 6 g L-1 Na+. The enriched planktonic communities were then used as inoculum for 3D printed, three-chamber cells equipped with a gas diffusion biocathode. The cells were fed with CO2 gas and operated galvanostatically (0.25 or 1.00 mA cm(-2)). The highest production rate of 55.4 g m(-2) d(-1) (0.89 g L-1 d(-1)), with 82.4% Coulombic efficiency, was obtained at 5 g L-1 NaCl concentration and 1 mA cm(-2) applied current, achieving an average acetate production of 44.7 kg MWh(-1). Scanning electron microscopy and 16S rRNA sequencing analysis confirmed the formation of a cathodic biofilm dominated by Acetobacterium sp. Finally, three 3D printed cells were hydraulically connected in series to simulate an MES stack, achieving three-fold production rates than with the single cell at 0.25 mA cm(-2). This confirms that three-chamber MES cells are an efficient and scalable technology for CO2 bio-electro recycling to acetate and that moderate saline conditions (5 g L-1 NaCl) can help reduce their power demand while preserving the activity of acetogens.(c) 2023 The Authors. Published by Elsevier B.V. on behalf of Chinese Society for Environmental Sciences, Harbin Institute of Technology, Chinese Research Academy of Environmental Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).