Internuclear gene silencing in Phytophthora infestans
Internuclear gene silencing in Phytophthora infestans
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DOI：
10.1016/s1097-2765(00)80461-x
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发表时间：
1999-03-01
期刊：
影响因子：
16
通讯作者：
Govers, F
中科院分区：
文献类型：
--
作者：
van West, P;Kamoun, S;Govers, F
转化的二倍体卵菌植物病原体致病疫霉与反义，正义，启动子少的结构的编码序列的激发素基因inf1导致转录沉默的转基因和内源基因。由于通过inf1沉默的转基因菌株和野生型菌株的体细胞融合获得的异核体显示稳定的基因沉默，inf1沉默是显性的，并在反式中起作用，Inf1在来自沉默异核体的非转基因同核后代中保持沉默，从而证明转基因的存在对于维持内源性inf1基因的沉默状态不是必需的。这些发现支持一个模型，让人想起副突变，并涉及一个反式作用因子，能够在细胞核之间转移沉默信号。
Transformation of the diploid oomycete plant pathogen Phytophthora infestans with antisense, sense, and promoter-less constructs of the coding sequence of the elicitin gene inf1 resulted in transcriptional silencing of both the transgenes and the endogenous gene. Since heterokaryons obtained by somatic fusion of an inf1-silenced transgenic strain and a wild-type strain displayed stable gene silencing, inf1 silencing is dominant and acts in trans, Inf1 remained silenced in nontransgenic homokaryotic progeny from the silenced heterokaryons, thereby demonstrating that the presence of transgenes is not essential for maintaining the silenced status of the endogenous inf1 gene. These findings support a model reminiscent of paramutation and involving a trans-acting factor that is capable of transferring a silencing signal between nuclei.