Effects of transplanted mesenchymal stem cells isolated from Wharton's jelly of caprine umbilical cord on cutaneous wound healing; histopathological evaluation
Effects of transplanted mesenchymal stem cells isolated from Wharton's jelly of caprine umbilical cord on cutaneous wound healing; histopathological evaluation
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DOI：
10.1007/s11259-011-9464-z
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发表时间：
2011-04-01
影响因子：
2.2
通讯作者：
Moshrefi, Mojgan
中科院分区：
文献类型：
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作者：
Azari, Omid;Babaei, Homayoon;Moshrefi, Mojgan
本研究旨在探讨羊脐带Wharton凝胶间充质干细胞(WJMSCs)移植对山羊皮肤创面愈合过程的影响。从屠宰场采集杂种山羊妊娠子宫，收获脐带华顿凝乳(WJ)。将组织切碎放入通风瓶中，采用组织块培养法分离间充质干细胞。对分离的细胞进行肌动蛋白免疫染色，组织化学检测碱性磷酸酶活性，并用流式细胞术分析基质受体(CD44)和造血祖细胞标志物(CD34)。分离细胞后，取3×10(6)骨髓间充质干细胞进行BrdU染色，准备移植到每个创面。在4只雷尼山羊胸椎两侧各做4个3 cm长的线性全厚皮肤切口(每侧2个伤口)。左侧创面在0.6ml磷酸盐缓冲液(PBS)中植入MSCs，右侧创面植入0.6ml磷酸盐缓冲液作为对照组。分别于伤后7天和12天取创面标本，对两组愈合过程进行组织学比较。抗BrdU染色显示，在研究过程中，移植的细胞在创面床上仍然活着。组织病理学检查显示，WJMSCs处理组创面在伤后7天已完全上皮化，而对照组创面伤后12天仍未完全上皮化。此外，镜下评估显示，与对照伤口相比，治疗创面炎症较少，肉芽组织形成较薄，疤痕最小。综上所述，本研究证实了山羊WJMSCs在山羊皮肤创面愈合中的有益作用。
The aim of this study was to investigate the effects of transplanted Wharton's jelly mesenchymal stem cells (WJMSCs) of caprine umbilical cord on cutaneous wound healing process in goat. After collection of caprine pregnant uterus of mixed breed goats from abattoir, the Wharton's jelly (WJ) of umbilical cord was harvested. The tissues were minced in ventilated flasks and explant culture method was used for separating mesenchymal stem cells (MSCs). The isolated cells were immunostained for Actin protein, histochemically assayed for the presence of alkaline phosphatase activity, and analyzed for detection of matrix receptors (CD44) and hematopoetic lineage markers (CD34), using flow cytometery. After The isolated cells, 3 x 10(6) MSCs were stained with BrdU and prepared for transplantation to each wound. Four 3-cm linear full thickness skin incisions were made on both sides of thoracic vertebrate of four Raeini goats (two wounds on each side). The left wounds were implanted with MSCs in 0.6 ml of Phosphate buffer saline (PBS), and the right wounds considered as control group that received 0.6 ml of PBS. The samples were taken from the wounds 7 and 12 days after the wounding, and healing process was compared histologically between the two groups. Anti-BrdU staining showed that the transplanted cells were still alive in the wound bed during the study. The histopathological study revealed that re-epithelialization was complete at days 7 in treated wounds with WJMSCs, whereas in control wound the wounds still showed incomplete epithelialization 12 days after wounding. Also, microscopic evaluation showed less inflammation, thinner granulation tissue formation with minimum scar in the treated wounds in comparison with control wounds. In conclusion, this study demonstrates the beneficial effect of caprine WJMSCs in cutaneous wound healing in goat.