Wall teichoic acid polymers are dispensable for cell viability in Bacillus subtilis
Wall teichoic acid polymers are dispensable for cell viability in Bacillus subtilis
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DOI：
10.1128/jb.01336-06
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发表时间：
2006-12-01
影响因子：
3.2
通讯作者：
Brown, Eric D.
中科院分区：
文献类型：
--
作者：
D'Elia, Michael A.;Millar, Kathryn E.;Brown, Eric D.
大量的文献已经确定，枯草芽孢杆菌中壁磷壁酸的合成对于细胞活力是必需的。有趣的是，我们最近发现壁磷壁酸生物合成在金黄色葡萄球菌（M。A. D'Elia，M. P.佩雷拉、Y. S. Chung，W. Zhao，中国粘蝇A.陈，T. J.肯尼，M. C. Sulavik，T. A. Black和E. D. Brown，J. Bacteriol. 188：4183-4189，2006）。在S.其中第一个基因（tarO）是显性的，随后的作用基因显示出不可或缺的表型。在这里，我们表明，为第一次，壁磷壁酸合成也在B。枯草芽孢杆菌中的一个类似的基因dispensability模式被视为后期作用的酶显示一个基本的表型，而基因tagO，其产物催化的途径中的第一步，可以被删除，以产生可行的突变体缺乏磷壁酸的细胞壁。
Am extensive literature has established that the synthesis of wall teichoic acid in Bacillus subtilis is essential for cell viability. Paradoxically, we have recently shown that wall teichoic acid biogenesis is dispensable in Staphylococcus aureus (M. A. D'Elia, M. P. Pereira, Y. S. Chung, W. Zhao, A. Chan, T. J. Kenney, M. C. Sulavik, T. A. Black, and E. D. Brown, J. Bacteriol. 188:4183-4189, 2006). A complex pattern of teichoic acid gene dispensability was seen in S. aureus where the first gene (tarO) was dispensable and later acting genes showed an indispensable phenotype. Here we show, for the first time, that wall teichoic acid synthesis is also dispensable in B. subtilis and that a similar gene dispensability pattern is seen where later acting enzymes display an essential phenotype, while the gene tagO, whose product catalyzes the first step in the pathway, could be deleted to yield viable mutants devoid of teichoic acid in the cell wall.