Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy
Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy
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DOI：
10.1021/acs.analchem.0c01800
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发表时间：
2020-11-17
影响因子：
7.4
通讯作者：
Watanabe, Tomonobu M.
中科院分区：
文献类型：
--
作者：
Germond, Arno;Panina, Yulia;Watanabe, Tomonobu M.
监测多能细胞中的细胞状态转变对于应用和基础研究是非常宝贵的。在这项研究中，我们证明了非侵入性的，无标记的拉曼光谱的相关性，以监测和表征小鼠干细胞进行重编程的细胞状态转换。使用小鼠干细胞的同基因细胞系，进行神经元细胞的重编程，我们展示了原始干细胞，其神经元祖细胞和重编程细胞的活单细胞光谱数据的比较分析。神经网络，回归模型和比率分析用于区分细胞状态，并提取几个重要的生物标志物特异性分化或重编程。我们的研究结果表明，拉曼光谱使我们能够建立一个低维空间，使我们能够监测和表征在单细胞水平上的细胞状态转变的动力学，分散在异质种群。通过拉曼光谱监测多能性和区分ES和重编程细胞之间的差异的能力也进行了讨论。
Monitoring cell-state transition in pluripotent cells is invaluable for application and basic research. In this study, we demonstrate the pertinence of noninvasive, label-free Raman spectroscopy to monitor and characterize the cell-state transition of mouse stem cells undergoing reprogramming. Using an isogenic cell line of mouse stem cells, reprogramming from neuronal cells was performed, and we showcase a comparative analysis of living single-cell spectral data of the original stem cells, their neuronal progenitors, and reprogrammed cells. Neural network, regression models, and ratiometric analyses were used to discriminate the cell states and extract several important biomarkers specific to differentiation or reprogramming. Our results indicated that the Raman spectrum allowed us to build a low-dimensional space allowing us to monitor and characterize the dynamics of cell-state transition at a single-cell level, scattered in heterogeneous populations. The ability of monitoring pluripotency by Raman spectroscopy and distinguishing differences between ES and reprogrammed cells is also discussed.