Development and validation of SNP genotyping assays to identify genetic sex in the swimming crab Portunus trituberculatus
Development and validation of SNP genotyping assays to identify genetic sex in the swimming crab Portunus trituberculatus
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DOI：
10.1016/j.aqrep.2021.100731
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发表时间：
2021-07
影响因子：
3.7
通讯作者：
Junkai Lu;Ronghua Li;Michaël Bekaert;H. Migaud;Xiao Liu;Qingwei Chen;Weiren Zhang;C. Mu;Weiwei Song;Chunlin Wang
中科院分区：
文献类型：
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作者：
Junkai Lu;Ronghua Li;Michaël Bekaert;H. Migaud;Xiao Liu;Qingwei Chen;Weiren Zhang;C. Mu;Weiwei Song;Chunlin Wang
三疣梭子蟹是中国和东南亚重要的经济水产养殖品种。由于市场价值的增加、消费者的偏好和增长业绩的增强，单性女性文化已被确定为重点优先事项。然而，缺乏快速可靠的性别连锁标记组阻碍了性别控制的应用和对物种性别决定的理解。在这项研究中，从我们简化的基因组数据中获得的两个性别连锁 SNP 通过测序进行了验证，然后转化为竞争性等位基因特异性 PCR 检测。结果显示遗传性别和表型性别之间具有高度一致性，因此提供了快速的性别鉴定测试。此外，雄性的异配基因型提供了重要的遗传证据来支持 P 中的 XX/XY 性别决定系统。三疣猴。
The swimming crabPortunus trituberculatusis an economically vital aquaculture species in China and South East Asia. Monosex female culture has been identified as a key priority due to their increased market value, consumer preference and enhanced growth performance. However, the lack of a rapid and reliable sex-linked marker panel has hampered the application of sex control and understanding of the sex determination in the species. In this study, two sex-linked SNPs obtained from our simplified genomic data were validated by sequencing and then converted into competitive allele-specific PCR assays. The results showed high consistency between the genetic and phenotypic sex providing therefore a rapid test sex identification. Moreover, heterogametic genotypes in males provided a significant genetic evidence to support an XX/XY sex determination system in theP. trituberculatus.