EXOGENOUS TRANSFORMING GROWTH-FACTOR-BETA AMPLIFIES ITS OWN EXPRESSION AND INDUCES SCAR FORMATION IN A MODEL OF HUMAN FETAL SKIN REPAIR
EXOGENOUS TRANSFORMING GROWTH-FACTOR-BETA AMPLIFIES ITS OWN EXPRESSION AND INDUCES SCAR FORMATION IN A MODEL OF HUMAN FETAL SKIN REPAIR
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DOI：
10.1097/00000658-199508000-00006
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发表时间：
1995-08-01
影响因子：
9
通讯作者：
ADZICK, NS
中科院分区：
文献类型：
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作者：
LIN, RY;SULLIVAN, KM;ADZICK, NS
目的胎儿皮肤伤口愈合不留疤痕。为了确定 IGF-β(1) 在胎儿伤口愈合中的作用，分析了人类胎儿和成人皮肤伤口中 TGF-β(1) 的 mRNA 表达。方法将人类胎儿皮肤移植到随后受伤的成年无胸腺小鼠的皮下位置，愈合后不会留下疤痕，而成人皮肤愈合时会在该位置形成疤痕。在成人伤口、胎儿伤口和用 TGF-β(1) 缓释盘处理的胎儿伤口中进行 TGF-β(1) mRNA 表达的原位杂交以及成纤维细胞、巨噬细胞和中性粒细胞的物种特异性免疫组织化学分析。结果损伤成人皮肤可诱导转化生长因子-β(1) mRNA 表达。在受伤后的人胎儿皮肤中未检测到 TGF-β(1) mRNA 上调。然而，当将外源性TGF-β(1)添加到人胎儿皮肤中时，诱导人胎儿成纤维细胞中TGF-β(1) mRNA表达，检测到类似成人的炎症反应，并且皮肤随着疤痕形成而愈合。结论转化生长因子-β(1)是疤痕形成的重要调节剂。抗 TGF-β(1) 策略可能会促进成人伤口的无疤痕愈合。
ObjectiveFetal skin wounds heal without scarring. To determine the role of IGF-beta(1) in fetal wound healing, mRNA expression of TGF-beta(1) was analyzed in human fetal and adult skin wounds.MethodsHuman fetal skin transplanted to a subcutaneous location on an adult athymic mouse that was subsequently wounded heals without scar, whereas human adult skin heals with scar formation in that location. In situ hybridization for TGF-beta(1) mRNA expression and species-specific immunohistochemistry for fibroblasts, macrophages, and neutrophils were performed in human adult wounds, fetal wounds, and fetal wounds treated with a TGF-beta(1) slow release disk.ResultsTransforming growth factor-beta(1) mRNA expression was induced by wounding adult skin. No TGF-beta(1) mRNA upregulation was detected in human fetal skin after wounding. However, when exogenous TGF-beta(1) was added to human fetal skin, induction of TGF-beta(1) mRNA expression in human fetal fibroblasts occurred, an adult-like inflammatory response was detected, and the skin healed with scar formation.ConclusionsTransforming growth factor-beta(1) is an important modulator in scar formation. Anti-TGF-beta(1) strategies may promote scarless healing in adult wounds.