The biosynthetic gene cluster for the β-lactam antibiotic tabtoxin in Pseudomonas syringae
The biosynthetic gene cluster for the β-lactam antibiotic tabtoxin in Pseudomonas syringae
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DOI：
10.1038/ja.2005.109
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发表时间：
2005-12-01
影响因子：
3.3
通讯作者：
Willis, DK
中科院分区：
文献类型：
--
作者：
Kinscherf, TG;Willis, DK
DNA序列分析表明，这种不常见的β-内酰胺类抗生素标签毒素的生物合成基因位于紫丁香假单胞菌BR2中lysC tRNA基因附近的att位点。该区域编码的ORF包括与β-内酰胺合成酶和克拉维酸合成酶相似的ORF，以及氨基酸合成酶。新的ORF存在于与毒素过敏性表型相关的生物合成区的一部分。烟草毒素抗性与一个主要的促进剂超家族(MFS)转运蛋白基因片段有关。
DNA sequence analysis revealed that the biosynthetic genes of the unusual beta-lactam antibiotic tabtoxin reside at the att site adjacent to the lysC tRNA gene in Pseudomonas syringae BR2. ORFs encoded within the region included ones with similarity to beta-lactam synthase and clavaminic acid synthase, as well as amino acid synthesis enzymes. Novel ORFs were present in a portion of the biosynthetic region associated with a toxin hypersensitivity phenotype. Tabtoxin resistance was associated with a fragment containing a major facilitator superfamily (MFS) transporter gene.